PC-12(低分化)细胞系

PC-12 （高分化）/PC-12 （高分化）细胞系/PC-12 （高分化）细胞株/PC-12 （高分化）大鼠肾上腺嗜铬细胞瘤细胞(高分化)

Cell line name PC12

Synonyms PC-12; PC 12; PC12.1

Accession CVCL_0481

Resource Identification Initiative To cite this cell line use: PC12 (RRID:CVCL_0481)

Comments Group: Space-flown cell line (cellonaut).

Characteristics: Responds reversibly to NGF by induction of a neuronal phenotype.

Doubling time: 40 hours (PubMed=9886495); 92 hours (CLS=500311; ECACC=88022401); ~50-60 hours (DSMZ=ACC-159).

Derived from site: In situ; Adrenal gland; UBERON=UBERON_0002369.

Disease Rat adrenal gland pheochromocytoma (NCIt: C121568)

Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)

Breed/subspecies: NEDH; RS=RS:0000603.

Hierarchy Children:

CVCL_0109 (123.7) CVCL_0133 (A126-1B2) CVCL_X983 (A208-4)

CVCL_X984 (A208-7) CVCL_X985 (AB.11) CVCL_YJ89 (LINTERNA PC-12)

CVCL_JY59 (Neuroscreen-1) CVCL_F659 (PC-12 Adh) CVCL_AU97 (PC-12 HD-Q23 cell 14)

CVCL_AU98 (PC-12 HD-Q23 cell 7) CVCL_AU99 (PC-12 HD-Q74 cell 10) CVCL_AV00 (PC-12 HD-Q74 cell 1b)

CVCL_5574 (PC-12TG) CVCL_C0PY (PC12 6c) CVCL_DN60 (PC12 HTT-SW2-Q145 clone 14)

CVCL_DN61 (PC12 HTT-SW2-Q145-TagRFP clone 5) CVCL_DN62 (PC12 HTT-SW2-Q23 clone 28) CVCL_DN63 (PC12 HTT-SW2-Q23-TagRFP clone 34)

CVCL_DN64 (PC12 HTT-SW2-Q73 clone 29) CVCL_DN65 (PC12 HTT-SW2-Q73-TagRFP clone 17) CVCL_V361 (PC12 Tet-Off)

CVCL_V333 (PC12 Tet-On) CVCL_C0PQ (PC12-1) CVCL_C0PR (PC12-15)

CVCL_C0PS (PC12-16A) CVCL_C0PT (PC12-19) CVCL_VH13 (PC12-1G2)

CVCL_C0PU (PC12-21) CVCL_C0PV (PC12-27) CVCL_VH10 (PC12-2B3)

CVCL_C130 (PC12-615) CVCL_J442 (PC12-AC) CVCL_VH11 (PC12-C3)

CVCL_VH12 (PC12-D60) CVCL_T752 (PC12-F7) CVCL_C0PX (PC12-FR)

CVCL_T753 (PC12-G11) CVCL_C4US (PC12-P1D10) CVCL_C4UT (PC12-P1F1)

CVCL_C0PP (PC12.4) CVCL_5573 (PC12.P3) CVCL_4681 (PC12D)

CVCL_JX88 (PC12h) CVCL_J438 (PC12HS) CVCL_J440 (PC12IS)

CVCL_4925 (PC12K) CVCL_J441 (PC12MS) CVCL_C126 (PC12nnr14)

CVCL_C127 (PC12nnr3) CVCL_C128 (PC12nnr5) CVCL_C129 (PC12nnr6)

CVCL_0483 (PC2 [Rat pheochromocytoma]) CVCL_7090 (PC4 [Rat pheochromocytoma]) CVCL_7091 (PC6)

CVCL_E1AL (Ubigene PC12 Gga3 KO) CVCL_E1AM (Ubigene PC12 Slc38a2 KO)

Sex of cell Male

Age at sampling Age unspecified

Category Cancer cell line

Publications

PubMed=1065897; DOI=10.1073/pnas.73.7.2424; PMCID=PMC430592

Greene L.A., Tischler A.S.

Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.

Proc. Natl. Acad. Sci. U.S.A. 73:2424-2428(1976)

 

PubMed=682602

Tischler A.S., Greene L.A.

Morphologic and cytochemical properties of a clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.

Lab. Invest. 39:77-89(1978)

 

PubMed=6500171; DOI=10.1016/0303-7207(84)90102-3

Bethea C.L., Kozak S.L.

Effect of extracellular matrix on PC 12 cell shape and dopamine processing.

Mol. Cell. Endocrinol. 37:319-329(1984)

 

PubMed=2493070; DOI=10.1111/j.1471-4159.1989.tb02538.x

Katoh-Semba R., Oohira A., Sano M., Watanabe K., Kitajima S., Kashiwamata S.

Glycosaminoglycan composition of PC12 pheochromocytoma cells: a comparison with PC12D cells, a new subline of PC12 cells.

J. Neurochem. 52:889-895(1989)

 

PubMed=2759161; DOI=10.1016/0277-5379(89)90388-X

Chelmicka-Schorr E., Checinski M.E., Arnason B.G.W.

PC12 pheochromocytoma and sympathetic nervous system derived trophic factors augment growth of neuroblastoma.

Eur. J. Cancer Clin. Oncol. 25:1057-1059(1989)

 

PubMed=2187480; DOI=10.1089/dna.1990.9.221

Muller S.R., Sullivan P.D., Clegg D.O., Feinstein S.C.

Efficient transfection and expression of heterologous genes in PC12 cells.

DNA Cell Biol. 9:221-229(1990)

 

PubMed=9886495; DOI=10.1002/(SICI)1097-4652(199901)178:1<93::AID-JCP12>3.0.CO;2-U

Dwyer D.S., Liu Y., Bradley R.J.

An ethanol-sensitive variant of the PC12 neuronal cell line: sensitivity to alcohol is associated with increased cell adhesion and decreased glucose accumulation.

J. Cell. Physiol. 178:93-101(1999)

 

PubMed=15541571; DOI=10.1016/j.mce.2003.02.001

Eaton M.J., Duplan H.

Useful cell lines derived from the adrenal medulla.

Mol. Cell. Endocrinol. 228:39-52(2004)

 

PubMed=16223334; DOI=10.1290/0411077.1

Dixon D.N., Loxley R.A., Barron A., Cleary S., Phillips J.K.

Comparative studies of PC12 and mouse pheochromocytoma-derived rodent cell lines as models for the study of neuroendocrine systems.

In Vitro Cell. Dev. Biol. Anim. 41:197-206(2005)

 

PubMed=18005394; DOI=10.1111/j.1748-1716.2007.01805.x; PMCID=PMC2663028

Westerink R.H.S., Ewing A.G.

The PC12 cell as model for neurosecretion.

Acta Physiol. 192:273-285(2008)

 

PubMed=22538498; DOI=10.1159/000338793

Grozinsky-Glasberg S., Shimon I., Rubinfeld H.

The role of cell lines in the study of neuroendocrine tumors.

Neuroendocrinology 96:173-187(2012)

 

PubMed=33171774; DOI=10.3390/ijms21218356; PMCID=PMC7664380

Kudo T.-a., Tominami K., Izumi S., Hayashi Y., Noguchi T., Matsuzawa A., Hong G., Nakai J.

Characterization of PC12 cell subclones with different sensitivities to programmed thermal stimulation.

Int. J. Mol. Sci. 21:8356.1-8356.15(2020)

 

PubMed=34348336; DOI=10.1159/000518337

Delage C., Breard-Mellin L., Theresine C., Simioneck S., Lefranc B., Leprince J., Benard M., Vaudry D.

The heterogeneity of response of PC12 cells from different laboratories to nerve growth factor and pituitary adenylate cyclase-activating polypeptide questions the reproducibility of studies carried out with tumor cell lines.

Neuroendocrinology 113:216-230(2023)