NCI-H157细胞系

NCI-H157、NCI-H157、NCI-H157细胞、NCI-H157细胞、NCI-H157人非小细胞肺鳞癌细胞

Cell line name NCI-H157

Synonyms NCl-H157; NCI H157; H157; H-157; NCI-157; NCIH157DM; H157DM

Accession CVCL_0463

Resource Identification Initiative To cite this cell line use: NCI-H157 (RRID:CVCL_0463)

Comments Problematic cell line: Contaminated. NCI-H157 and NCI-H1264 have been shown to be identical.

Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00412.

Doubling time: 53 hours (Note=In RPMI 1640 + 10% FBS), 90 hours (Note=In ACL-3 + BSA) (PubMed=3940644); 20.8 hours (PubMed=29681454).

Omics: Array-based CGH.

Omics: Deep exome analysis.

Omics: Deep proteome analysis.

Omics: miRNA expression profiling.

Omics: Protein expression by reverse-phase protein arrays.

Omics: SNP array analysis.

Omics: Transcriptome analysis by microarray.

Sequence variations

Mutation; HGNC; 1787; CDKN2A; Simple; p.His83Tyr (c.247C>T) (p.Ala97Val, c.290C>T); ClinVar=VCV000376307; Zygosity=Homozygous (PubMed=30038707; DepMap=ACH-000921).

Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Arg (c.34G>C); ClinVar=VCV000012579; Zygosity=Heterozygous (PubMed=1311061; PubMed=12068308; DepMap=ACH-000921).

Mutation; HGNC; 11100; SMARCA4; Simple; p.Thr58Profs*36 (c.171del); Zygosity=Homozygous (DepMap=ACH-000921).

Mutation; HGNC; 11998; TP53; Simple; p.Leu35Phefs*8 (c.102_103insT) (c.103_104insT); Zygosity=Heterozygous (PubMed=10536175; PubMed=20557307; DepMap=ACH-000921).

Mutation; HGNC; 11998; TP53; Simple; p.Glu298Ter (c.892G>T); ClinVar=VCV000093323; Zygosity=Heterozygous (PubMed=1311061; PubMed=10536175; PubMed=20557307; DepMap=ACH-000921).

Disease Lung squamous cell carcinoma (NCIt: C3493)

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_VD88 (H157R)

Originate from same individual CVCL_3970 ! NCI-H1264

Sex of cell Male

Age at sampling 59Y

Category Cancer cell line

STR profile Source(s): ATCC=CRL-5802; CCRID=1101HUM-PUMC000113; CLS=300387

 

Markers:

Amelogenin X

CSF1PO 12

D2S1338 21,22

D3S1358 17,18

D5S818 10,13

D6S1043 17,24

D7S820 12

D8S1179 14,16

D12S391 20

D13S317 12

D16S539 12,13

D18S51 13,15

D19S433 11,13

D21S11 32

FGA 22,23

Penta D 2.2

Penta E 7

TH01 7,9

TPOX 6,12

vWA 15

 

Run an STR similarity search on this cell line

PubMed=6270685; DOI=10.1073/pnas.78.7.4591; PMCID=PMC319839

Cuttitta F., Rosen S.T., Gazdar A.F., Minna J.D.

Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.

Proc. Natl. Acad. Sci. U.S.A. 78:4591-4595(1981)

 

PubMed=6272398; DOI=10.1126/science.6272398

Moody T.W., Pert C.B., Gazdar A.F., Carney D.N., Minna J.D.

High levels of intracellular bombesin characterize human small-cell lung carcinoma.

Science 214:1246-1248(1981)

 

PubMed=6148444; DOI=10.1093/jnci/73.4.801

Morstyn G., Russo A., Carney D.N., Karawya E., Wilson S.H. Jr., Mitchell J.B.

Heterogeneity in the radiation survival curves and biochemical properties of human lung cancer cell lines.

J. Natl. Cancer Inst. 73:801-807(1984)

 

PubMed=6713399

Rosen S.T., Mulshine J.L., Cuttitta F., Fedorko J., Carney D.N., Gazdar A.F., Minna J.D.

Analysis of human small cell lung cancer differentiation antigens using a panel of rat monoclonal antibodies.

Cancer Res. 44:2052-2061(1984)

 

PubMed=3940644

Brower M., Carney D.N., Oie H.K., Gazdar A.F., Minna J.D.

Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium.

Cancer Res. 46:798-806(1986)

 

PubMed=1311061

Mitsudomi T., Steinberg S.M., Nau M.M., Carbone D.P., D'Amico D., Bodner S.M., Oie H.K., Linnoila R.I., Mulshine J.L., Minna J.D., Gazdar A.F.

p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features.

Oncogene 7:171-180(1992)

 

PubMed=1563005

Giaccone G., Battey J., Gazdar A.F., Oie H.K., Draoui M., Moody T.W.

Neuromedin B is present in lung cancer cell lines.

Cancer Res. 52:2732s-2736s(1992)

 

PubMed=7972006; DOI=10.1073/pnas.91.23.11045; PMCID=PMC45163

Okamoto A., Demetrick D.J., Spillare E.A., Hagiwara K., Hussain S.P., Bennett W.P., Forrester K., Gerwin B.I., Serrano M., Beach D.H., Harris C.C.

Mutations and altered expression of p16INK4 in human cancer.

Proc. Natl. Acad. Sci. U.S.A. 91:11045-11049(1994)

 

PubMed=8626706; DOI=10.1074/jbc.271.19.11477

Quinn K.A., Treston A.M., Unsworth E.J., Miller M.-J., Vos M., Grimley C., Battey J., Mulshine J.L., Cuttitta F.

Insulin-like growth factor expression in human cancer cell lines.

J. Biol. Chem. 271:11477-11483(1996)

 

PubMed=8806092; DOI=10.1002/jcb.240630505

Phelps R.M., Johnson B.E., Ihde D.C., Gazdar A.F., Carbone D.P., McClintock P.R., Linnoila R.I., Matthews M.J., Bunn P.A. Jr., Carney D.N., Minna J.D., Mulshine J.L.

NCI-Navy Medical Oncology Branch cell line data base.

J. Cell. Biochem. Suppl. 24:32-91(1996)