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T25
293T细胞293T人胚肾细胞
Cell line name HEK293T
Synonyms Hek293T; HEK-293T; HEK 293T; HEK-293-T; HEK 293 T; 293-T; 293 T; 293T; Human Embryonic Kidney 293T; 293tsA1609neo
Accession CVCL_0063
Resource Identification Initiative To cite this cell line use: HEK293T (RRID:CVCL_0063)
Comments Part of: ENCODE project common cell types; tier 3.
Part of: MD Anderson Cell Lines Project.
Virology: Highly susceptible to infection by Zika virus (ZIKV) (PubMed=29468137).
Doubling time: ~24-30 hours (DSMZ=ACC-635).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Omics: Deep proteome analysis.
Omics: Genome sequenced.
Omics: miRNA expression profiling.
Omics: Mitochondrial proteome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: Transcriptome analysis by RNAseq.
Misspelling: HECK293T; Note=Occasionally.
Misspelling: HEK 239T; Note=Occasionally.
Misspelling: HEK239T; Note=Occasionally.
Misspelling: HEK-393T; Note=Occasionally.
Misspelling: HEK393T; Note=Occasionally.
Misspelling: 239T; Note=Occasionally.
Misspelling: 393T; Note=Occasionally.
Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
PubMed=3031469; DOI=10.1128/mcb.7.1.379-387.1987; PMCID=PMC365079
DuBridge R.B., Tang P., Hsia H.-C., Leong P.-M., Miller J.H., Calos M.P.
Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system.
Mol. Cell. Biol. 7:379-387(1987)
PubMed=15900046; DOI=10.1093/jnci/dji133
Mashima T., Oh-hara T., Sato S., Mochizuki M., Sugimoto Y., Yamazaki K., Hamada J.-i., Tada M., Moriuchi T., Ishikawa Y., Kato Y., Tomoda H., Yamori T., Tsuruo T.
p53-defective tumors with a functional apoptosome-mediated pathway: a new therapeutic target.
J. Natl. Cancer Inst. 97:765-777(2005)
PubMed=25182477; DOI=10.1038/ncomms5767; PMCID=PMC4166678
Lin Y.-C., Boone M., Meuris L., Lemmens I., Van Roy N., Soete A., Reumers J., Moisse M., Plaisance S., Drmanac R., Chen J., Speleman F., Lambrechts D., Van de Peer Y., Tavernier J., Callewaert N.
Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.
Nat. Commun. 5:4767.1-4767.12(2014)
PubMed=26694163; DOI=10.1371/journal.pone.0144924; PMCID=PMC4692263
Janiszewska J., Szaumkessel M., Kostrzewska-Poczekaj M., Bednarek K., Paczkowska J., Jackowska J., Grenman R., Szyfter K., Wierzbicka M., Giefing M., Jarmuz-Szymczak M.
Global miRNA expression profiling identifies miR-1290 as novel potential oncomiR in laryngeal carcinoma.
PLoS ONE 10:E0144924-E0144924(2015)
PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
PubMed=28601559; DOI=10.1016/j.cels.2017.05.009; PMCID=PMC5493283
Bekker-Jensen D.B., Kelstrup C.D., Batth T.S., Larsen S.C., Haldrup C., Bramsen J.B., Sorensen K.D., Hoyer S., Orntoft T.F., Lindbjerg Andersen C., Nielsen M.L., Olsen J.V.
An optimized shotgun strategy for the rapid generation of comprehensive human proteomes.
Cell Syst. 4:587-599.e4(2017)
DOI=10.13005/bpj/1414
Yuan J., Xu W.W.-Y., Jiang S.Y.-L., Yu H.X.-Y., Poon H.-F.
The scattered twelve tribes of HEK293.
Biomed. Pharmacol. J. 11:621-623(2018)
PubMed=29468137; DOI=10.5501/wjv.v7.i1.10; PMCID=PMC5807893
Himmelsbach K., Hildt E.
Identification of various cell culture models for the study of Zika virus.
World J. Virol. 7:10-20(2018)
PubMed=34800366; DOI=10.1016/j.cmet.2021.11.001; PMCID=PMC8664129
Morgenstern M., Peikert C.D., Lubbert P., Suppanz I., Klemm C., Alka O., Steiert C., Naumenko N., Schendzielorz A., Melchionda L., Muhlhauser W.W.D., Knapp B., Busch J.D., Stiller S.B., Dannenmaier S., Lindau C., Licheva M., Eickhorst C., Galbusera R., Zerbes R.M., Ryan M.T., Kraft C., Kozjak-Pavlovic V., Drepper F., Dennerlein S., Oeljeklaus S., Pfanner N., Wiedemann N., Warscheid B.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Cell Metab. 33:2464-2483.e18(2021)
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文献和实验*发表【中文论文】请标注：由博辉生物科技（广州）有限公司提供； *发表【英文论文】请标注：From Bohui Biological Technology (Guangzhou) Co., Ltd.
edwardellen 求助！！！ 本人菜鸟，最近开始接触凋亡相关信号，在HepG-2中做实验，有一点很困惑，HepG-2中的p53是野生型的还是突变型的？还是两者都存在？查了一些资料，有些文章之间表达的意思模凌两可。困惑啊！ 哪位好心的达人能给我一个确定的答案呢？ 另外，是否有做p53相关的牛人能告诉我P53在哪些实验用肿瘤细胞系中是野生型的，在哪些是突变型的？ 谢谢！谢谢！谢谢！ doctormy
shao74 最近在做细胞凋亡方面的实验,碰到的问题是手头上的细胞系对凋亡不是很敏感,所以不知哪位有更好的建议,谢谢! freecell 这个不好说啊，看你主要做哪个组织的细胞凋亡,什么因素引起的细胞凋亡。 Fasta921 所以要自己筛选敏感的细胞株啊，不同细胞系对同种基因或药物的耐受性不一样的。 goldendoctor Hela 是比较常用
Transient transfection into 293T cells
Purpose Transient transfection into 293T cells is a convenient way to overexpress and obtain both cellular and extracellular (secreted or membrane) proteins. 293 is a human renal epithelial cell line which is transformed by adenovirus E1
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