HET-1A细胞

Het-1A/Het-1A细胞系/Het-1A细胞株/Het-1A人食管上皮细胞

Cell line name HET-1A

Synonyms Het-1A; HET1A; Het1A

Accession CVCL_3702

Resource Identification Initiative To cite this cell line use: HET-1A (RRID:CVCL_3702)

Comments Group: Patented cell line.

Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10209.

Population: African American.

Doubling time: 27.4 +- 3.6 hours (PubMed=21127259).

Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRSV-T).

Omics: Transcriptome analysis by microarray.

Omics: Transcriptome analysis by RNAseq.

Caution: Indicated as originating from a 25 year old male in ATCC, but said to be derived from autopsy specimen from a 74 year old male.

Derived from site: In situ; Esophagus; UBERON=UBERON_0001043.

Cell type: Epithelial cell of esophagus; CL=CL_0002252.

Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy Children:

CVCL_4T84 (HET1AR)

Sex of cell Male

Age at sampling 74Y

Category Transformed cell line

STR profile Source(s): ATCC=CRL-2692

 

Markers:

Amelogenin X,Y

CSF1PO 10,12

D2S1338 17,23

D3S1358 15,17

D5S818 11,12

D7S820 9

D8S1179 13,14

D13S317 11

D16S539 9,11

D18S51 14

D19S433 12,14

D21S11 28,31

FGA 22,25

Penta D 12,13

Penta E 7

TH01 7

TPOX 11

vWA 16

 

Run an STR similarity search on this cell line

Publications

CLPUB00265

Stoner G.D., Kaighn M.E., Resau J.H., Naito Z., Bowman D., Harris C.C.

Immortalized human esophageal epithelial cell lines for studies of carcinogen-induced cell transformation.

In Vitro Cell. Dev. Biol. 26:23A-23A(1990)

 

DOI=10.1007/978-1-4612-0411-4_30

Stoner G.D., Light B.A., Harris C.C.

Immortalization and oncogene transformation of human esophageal epithelial cells.

(In book chapter) Neoplastic transformation in human cell system. Mechanism of carcinogenesis; Rhim J.S., Dritschilo A. (eds.); pp.297-305; Humana Press; New York; USA (1990)

 

Patent=WO1991005062A1

Stoner G.D., Reddel R.R., Harris C.C.

Human esophageal epithelial cell lines.

Patent number WO1991005062A1, 18-Apr-1991

 

PubMed=1703038

Stoner G.D., Kaighn M.E., Reddel R.R., Resau J.H., Bowman D., Naito Z., Matsukura N., You M., Galati A.J., Harris C.C.

Establishment and characterization of SV40 T-antigen immortalized human esophageal epithelial cells.

Cancer Res. 51:365-371(1991)

 

PubMed=8504475; DOI=10.1093/carcin/14.5.833

Lehman T.A., Modali R.V., Boukamp P., Stanek J., Bennett W.P., Welsh J.A., Metcalf R.A., Stampfer M.R., Fusenig N.E., Rogan E.M., Harris C.C.

p53 mutations in human immortalized epithelial cell lines.

Carcinogenesis 14:833-839(1993)

 

PubMed=7972006; DOI=10.1073/pnas.91.23.11045; PMCID=PMC45163

Okamoto A., Demetrick D.J., Spillare E.A., Hagiwara K., Hussain S.P., Bennett W.P., Forrester K., Gerwin B.I., Serrano M., Beach D.H., Harris C.C.

Mutations and altered expression of p16INK4 in human cancer.

Proc. Natl. Acad. Sci. U.S.A. 91:11045-11049(1994)

 

PubMed=10078940; DOI=10.1002/(SICI)1098-2744(199902)24:2<128::AID-MC7>3.0.CO;2-F

Compton K.R., Orringer M.B., Beer D.G.

Induction of glutathione s-transferase-pi in Barrett's metaplasia and Barrett's adenocarcinoma cell lines.

Mol. Carcinog. 24:128-136(1999)

 

PubMed=20139130; DOI=10.1093/carcin/bgq036

Duggan S.P., Behan F.M., Kirca M., Smith S.M., Reynolds J.V., Long A., Kelleher D.P.

An integrative genomic approach in oesophageal cells identifies TRB3 as a bile acid responsive gene, downregulated in Barrett's oesophagus, which regulates NF-kappaB activation and cytokine levels.

Carcinogenesis 31:936-945(2010)

 

PubMed=20843300; DOI=10.1042/BC20100071

Underwood T.J., Derouet M.F., White M.J., Noble F., Moutasim K.A., Smith E., Drew P.A., Thomas G.J., Primrose J.N., Blaydes J.P.

A comparison of primary oesophageal squamous epithelial cells with HET-1A in organotypic culture.

Biol. Cell 102:635-644(2010)

 

PubMed=21127259; DOI=10.1152/ajpgi.00461.2010; PMCID=PMC3043651

Goldman A., Chen H.D.R., Roesly H.B., Hill K.A., Tome M.E., Dvorak B., Bernstein H., Dvorak K.

Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis.

Am. J. Physiol. Gastrointest. Liver Physiol. 300:G292-G302(2011)

 

DOI=10.1155/2014/376541

Akagi I., Ishibashi O., Matsutani T., Hagiwara N., Matsuda A., Nomura T., Makino H., Yoshida H., Miyashita M., Uchida E.

Comprehensive analysis of microRNA and mRNA expression in normal and tumorous human esophageal squamous cell lines using microarray datasets.

Dataset Papers Sci. 2013:376541.1-376541.3(2013)

 

PubMed=28224081; DOI=10.1016/j.gdata.2017.02.002; PMCID=PMC5304241

Akagi I., Ishibashi O.

RNA-Seq analysis of human cell lines established from normal and neoplastic esophageal squamous epithelium.

Genom. Data 12:4-6(2017)