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- 2025年12月15日
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T25
Sp2/0-Ag14/Sp2/0-Ag14细胞系/Sp2/0-Ag14细胞株/Sp2/0-Ag14小鼠骨髓瘤细胞
Cell line name Sp2/0-Ag14
Synonyms SP2/0-Ag14; SP2/0-AG14; SP2/0-ag14; Sp2/O-Ag14; SP2/O-Ag14; Sp2/0-Ag-14; SP2-0-Ag14; SP2/0 Ag-14; SP-2/0-AG14; Sp 2/0-Ag 14; Sp2/0; SP2/0; Sp2/O; SP2/O; SP-2; SP2; GM03569; GM3569; GM03569B; GM3569B; GM03569D
Accession CVCL_2199
Resource Identification Initiative To cite this cell line use: Sp2/0-Ag14 (RRID:CVCL_2199)
Comments Group: Hybridoma fusion partner cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-8287.
Doubling time: ~30-40 hours (DSMZ=ACC-146).
Selected for resistance to: ChEBI; CHEBI:63486; 8-azaguanine.
Misspelling: SP2/0-Ag15; Note=Occasionally.
Misspelling: Sp20; PubMed=25723277.
Disease Mouse plasma cell myeloma (NCIt: C125417)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
PubMed=714156; DOI=10.1038/276269a0
Shulman M., Wilde C.D., Kohler G.J.F.
A better cell line for making hybridomas secreting specific antibodies.
Nature 276:269-270(1978)
PubMed=6769123; DOI=10.1073/pnas.77.4.2197; PMCID=PMC348679
Kohler G.J.F.
Immunoglobulin chain loss in hybridoma lines.
Proc. Natl. Acad. Sci. U.S.A. 77:2197-2199(1980)
CLPUB00447
Mulivor R.A., Suchy S.F.
1992/1993 catalog of cell lines. NIGMS human genetic mutant cell repository. 16th edition. October 1992.
(In misc. document) Institute for Medical Research (Camden, N.J.) NIH 92-2011; pp.1-918; National Institutes of Health; Bethesda; USA (1992)
PubMed=1373425; DOI=10.1016/0022-1759(92)90173-q
Harris J.F., Hawley R.G., Hawley T.S., Crawford-Sharpe G.C.
Increased frequency of both total and specific monoclonal antibody producing hybridomas using a fusion partner that constitutively expresses recombinant IL-6.
J. Immunol. Methods 148:199-207(1992)
PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)
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文献和实验*发表【中文论文】请标注：由博辉生物科技（广州）有限公司提供； *发表【英文论文】请标注：From Bohui Biological Technology (Guangzhou) Co., Ltd.
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