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- BHcell（博辉生物）
- BH-C419
- 2025年12月24日
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T25
LLC/LLC细胞系/LLC细胞株/LLC小鼠肺癌细胞
Cell line name LL/2 (LLC1)
Synonyms LL/2 (LLc1); LL/2(LLc1); LL/2; LL2; LLC1; LLC; Lewis lung carcinoma line 1; Lewis lung carcinoma; Lewis Lung Cancer; Lewis-Lung; Lewis Lung
Accession CVCL_4358
Resource Identification Initiative To cite this cell line use: LL/2 (LLC1) (RRID:CVCL_4358)
Comments Doubling time: 21 hours (CLS=400263).
Omics: HLA class I peptidome analysis by proteomics.
Omics: Transcriptome analysis by microarray.
Caution: Could be identical to 3LL (Cellosaurus=CVCL_5653).
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Disease Malignant tumors of the mouse pulmonary system (NCIt: C21769)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Children:
CVCL_E1LR (HyCyte LL/2 (LLC1) KO-mIrf7) CVCL_D6BW (HyCyte LL/2 (LLC1) KO-mKeap1) CVCL_D6BX (HyCyte LL/2 (LLC1) KO-mMdm4)
CVCL_D6BY (HyCyte LL/2 (LLC1) KO-mMif) CVCL_E1LS (HyCyte LL/2 (LLC1) KO-mSting1) CVCL_RA22 (LL/2-eGFP-Puro)
CVCL_RA23 (LL/2-Fluc-Neo/eGFP-Puro) CVCL_RA24 (LL/2-Fluc-Puro) CVCL_RA25 (LL/2-iRFP-Puro)
CVCL_5J40 (LL/2-luc-M38) CVCL_A4CM (LL/2-Luc2) CVCL_RA26 (LL/2-mNIS (monoclonal))
CVCL_RA27 (LL/2-mNIS-Neo/eGFP-Puro) CVCL_RA28 (LL/2-mNIS-Neo/Fluc-Puro) CVCL_RA29 (LL/2-mNIS-Puro)
CVCL_5J02 (LL/2-Red-FLuc) CVCL_H949 (LLAK) CVCL_H948 (LLTC)
CVCL_E0X9 (Ubigene LLC Keap1 KO) CVCL_E0XA (Ubigene LLC Nadk2 KO)
Category Cancer cell line
STR profile Source(s): PubMed=31220119; Ubigene=YC-C018
Markers:
Mouse STR 1-1 15,17,18
Mouse STR 1-2 18,19,20
Mouse STR 2-1 15,16
Mouse STR 3-2 13,14
Mouse STR 4-2 20.3,21.3,23.3 (PubMed=31220119)
21.3,23.3 (Ubigene=YC-C018)
Mouse STR 5-5 16,17,18 (PubMed=31220119)
16,17 (Ubigene=YC-C018)
Mouse STR 6-4 17,18 (PubMed=31220119)
18 (Ubigene=YC-C018)
Mouse STR 6-7 14,15,16 (PubMed=31220119)
15 (Ubigene=YC-C018)
Mouse STR 7-1 26.2,27.2,28.2
Mouse STR 8-1 16
Mouse STR 11-2 13.3,14.3,15.3,16
Mouse STR 12-1 17
Mouse STR 13-1 17,18
Mouse STR 15-3 22.3,24.3 (PubMed=31220119)
24.3,25.3 (Ubigene=YC-C018)
Mouse STR 17-2 14,15,16
Mouse STR 18-3 15,16
Mouse STR 19-2 13
Mouse STR X-1 24,26,28 (PubMed=31220119)
25,25.3 (Ubigene=YC-C018)
Run an STR similarity search on this cell line
Publications
PubMed=7226139; DOI=10.1016/0304-3835(80)90130-5
Bertram J.S., Janik P.
Establishment of a cloned line of Lewis lung carcinoma cells adapted to cell culture.
Cancer Lett. 11:63-73(1980)
DOI=10.4051/ibc.2010.2.4.0014
Yoshino K., Saijo K., Noro C., Nakamura Y.
Development of a simple method to determine the mouse strain from which cultured cell lines originated.
Interdisciplinary Bio Central 2:1-9(2010)
PubMed=28599420; DOI=10.3892/ol.2017.5877; PMCID=PMC5453008
Stankevicius V., Kuodyte K., Schveigert D., Bulotiene D., Paulauskas T., Daniunaite K., Suziedelis K.
Gene and miRNA expression profiles of mouse Lewis lung carcinoma LLC1 cells following single or fractionated dose irradiation.
Oncol. Lett. 13:4190-4200(2017)
PubMed=30084848; DOI=10.1038/sdata.2018.157; PMCID=PMC6080492
Schuster H., Shao W.-G., Weiss T., Pedrioli P.G.A., Roth P., Weller M., Campbell D.S., Deutsch E.W., Moritz R.L., Planz O., Rammensee H.-G., Aebersold R., Caron E.
A tissue-based draft map of the murine MHC class I immunopeptidome.
Sci. Data 5:180157-180157(2018)
PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)
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文献和实验*发表【中文论文】请标注：由博辉生物科技（广州）有限公司提供； *发表【英文论文】请标注：From Bohui Biological Technology (Guangzhou) Co., Ltd.
LLC-RK1 Cell Screening Test for Nephrotoxicity
In this test, kidney-derived cells are cultured in the presence of test compounds whose cytotoxicity is then determined by the Neutral Red method, and serves as an indicator of potential nephrotoxicity (1 ). Healthy LLC-RK1 cells
我和师兄是从04年初就开始用LLC-PK1细胞系筛选多种类型的、数量极多的各种可能有用的新药，摸索发现消化液的配方为0.25％的胰酶+0.02%EDTA 就行了，要配在PBS中，如果能加Hepes那就更保险了（有一次实验室Hepes用光了，配液时没加Hepes，照样没问题） 消化步骤：吸走培液后最好用PBS过一遍－>50ML培养瓶加2ML在37ºC预热消化液－>37ºC温箱放置1Min－>显微镜下观察，细胞松散开，但尚未脱落时，及时吸走消化液－>必要时可在吸走消化液的情况下，继续放置一会
细胞治疗是细胞和基因治疗的重要组成部分，它通过使用特定类型的细胞来修复、替换或调节受损的组织和器官。在细胞治疗中，不同类型的细胞因其独特的生物学特性而被广泛应用于多种疾病的治疗。以下是一些常用的细胞类型及其在细胞治疗中的应用。 （1）免疫细胞 免疫细胞是细胞治疗中最重要且研究最多的细胞类型之一，主要包括 T 细胞、自然杀伤细胞（NK 细胞）和树突状细胞（DC 细胞）。 T 细胞：T 细胞是人体免疫系统的核心细胞，具有强大的抗肿瘤能力。CAR-T 细胞疗法是目前最成功的免疫细胞治疗技术
技术资料