VERO细胞

Vero/Vero细胞系/Vero细胞株/Vero非洲绿猴正常肾细胞

Cell line name Vero

Synonyms VERO; VeroCCL81; Vero 81; Vero-81; Verda reno

Accession CVCL_0059

Resource Identification Initiative To cite this cell line use: Vero (RRID:CVCL_0059)

Comments Group: Non-human primate cell line.

Group: Vaccine production cell line.

Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).

Biotechnology: Used for the production of the vaccine (Trade name: IXIARO/JESPECT).

Biotechnology: Used for the production of the inactivated poliovirus vaccine (Trade name: Imovax Polio).

Biotechnology: Used for the production of the Purified Vero-cell Rabies Vaccine (PVRV) (Trade name: VERORAB).

Biotechnology: Used for the production of the Chromatographically Purified Rabies Vaccine (CPRV).

Biotechnology: Used for the production of the live oral rotavirus vaccine (Trade Name: Rotarix).

Biotechnology: Used for the production of the live smallpox (Vaccinia) vaccine (Trade name: ACAM2000).

Biotechnology: Used for the production of the tetravalent dengue vaccine (Trade Name: Dengvaxia).

Biotechnology: Used for the production of the Bharat Biotech SARS-CoV-2 (COVID-19) vaccine (Trade name: Covaxin; also known as BBV152) (DrugBank=DB15847). The cell line is used to produces a inactivated SARS-CoV-2 virus strain.

Biotechnology: Used for the production of the Sinovax SARS-CoV-2 (COVID-19) vaccine (Trade name: CoronaVac; also known as PiCoVacc) (DrugBank=DB15806). The cell line is used to produces the inactivated SARS-CoV-2 virus strain CN02 (PubMed=32376603).

Virology: Susceptible to infection by SARS coronavirus (SARS-CoV). Produces a lytic infection (PubMed=15731278; PubMed=16494729).

Virology: Susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19). But Vero C1008 (often known as Vero E6) (Cellosaurus=CVCL_0574) seems to be more appropriate for amplification and quantification (PubMed=32511316; PubMed=33389257).

Omics: Genome sequenced.

Anecdotal: Verda reno means green kidney in Esperanto and Vero means truth also in Esperanto.

Derived from site: In situ; Kidney, epithelium; UBERON=UBERON_0004819.

Cell type: Epithelial cell of kidney; CL=CL_0002518.

PubMed=10494966

Montagnon B.J., Vincent-Falquet J.-C., Saluzzo J.-F.

Experience with Vero cells at Pasteur Merieux Connaught.

Dev. Biol. Stand. 98:137-140(1999)

 

PubMed=12667817; DOI=10.1016/S0042-6822(02)00064-8

Romanova J., Katinger D., Ferko B., Voglauer R., Mochalova L., Bovin N., Lim W., Katinger H., Egorov A.

Distinct host range of influenza H3N2 virus isolates in Vero and MDCK cells is determined by cell specific glycosylation pattern.

Virology 307:90-97(2003)

 

PubMed=15731278; DOI=10.1128/JVI.79.6.3846-3850.2005; PMCID=PMC1075706

Mossel E.C., Huang C., Narayanan K., Makino S., Tesh R.B., Peters C.J.

Exogenous ACE2 expression allows refractory ce

J. Virol. 79:3846-3850(2005)

 

PubMed=16494729; DOI=10.3201/eid1201.050496; PMCID=PMC3291385

Kaye M., Druce J., Tran T., Kostecki R., Chibo D., Morris J., Catton M., Birch C.

SARS-associated coronavirus replication in cell lines.

Emerg. Infect. Dis. 12:128-133(2006)

 

DOI=10.12665/J63.Morgan

Morgan J.H.

Vero cells in vaccine production.

BioProcess. J. 6:12-17(2007)

 

PubMed=19016439; DOI=10.1002/9780471729259.mca04es11; PMCID=PMC2657228

Ammerman N.C., Beier-Sexton M., Azad A.F.

Growth and maintenance of Vero cell lines.

Curr. Protoc. Microbiol. 11:A.4E.1-A.4E.7(2008)

 

PubMed=19768803; DOI=10.1002/btpr.279

Rourou S., van der Ark A., van der Velden T., Kallel H.

Development of an animal-component free medium for Vero cells culture.

Biotechnol. Prog. 25:1752-1761(2009)

 

PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022

Karger A., Bettin B., Lenk M., Mettenleiter T.C.

Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.

J. Virol. Methods 164:116-121(2010)

 

PubMed=22059503; DOI=10.1186/1472-6750-11-102; PMCID=PMC3221628

Almeida J.L., Hill C.R., Cole K.D.

Authentication of African green monkey cell lines using human short tandem repeat markers.

BMC Biotechnol. 11:102.1-102.10(2011)

 

PubMed=24975811; DOI=10.1016/j.vaccine.2014.06.045; PMCID=PMC5915289

Donis R.O., Chen L.-M., Davis C.T., Foust A., Hossain M.J., Johnson A., Klimov A., Loughlin R.F., Xu X.-Y., Tsai T., Blayer S., Trusheim H., Colegate T., Fox J., Taylor B., Hussain A.I., Barr I., Baas C., Louwerens J., Geuns E., Lee M.-S., Venhuizen L., Neumeier E., Ziegler T.

Influenza Cell Culture Working Group

Performance characteristics of qualified cell lines for isolation and propagation of influenza viruses for vaccine manufacturing.

Vaccine 32:6583-6590(2014)

 

PubMed=25267831; DOI=10.1093/dnares/dsu029; PMCID=PMC4263300

Osada N., Kohara A., Yamaji T., Hirayama N., Kasai F., Sekizuka T., Kuroda M., Hanada K.

The genome landscape of the African green monkey kidney-derived Vero cell line.

DNA Res. 21:673-683(2014)

 

PubMed=25903999; DOI=10.1002/biot.201400388

Genzel Y.

Designing cell lines for viral vaccine production: where do we stand?

Biotechnol. J. 10:728-740(2015)

 

PubMed=28386771; DOI=10.1007/s10616-017-0082-7; PMCID=PMC5595746

Andreani N.A., Renzi S., Piovani G., Ajmone-Marsan P., Bomba L., Villa R., Ferrari M., Dotti S.

Potential neoplastic evolution of Vero cells: in vivo and in vitro characterization.

Cytotechnology 69:741-750(2017)

 

PubMed=32376603; DOI=10.1126/science.abc1932; PMCID=PMC7202686

Gao Q., Bao L.-L., Mao H.-Y., Wang L., Xu K.-W., Yang M.-N., Li Y.-J., Zhu L., Wang N., Lv Z., Gao H., Ge X.-Q., Kan B., Hu Y.-L., Liu J.-N., Cai F., Jiang D.-Y., Yin Y.-H., Qin C.-F., Li J., Gong X.-J., Lou X.-Y., Shi W., Wu D.-D., Zhang H.-M., Zhu L., Deng W., Li Y.-R., Lu J.-X., Li C.-G., Wang X.-X., Yin W.-D., Zhang Y.-J., Qin C.

Development of an inactivated vaccine candidate for SARS-CoV-2.

Science 369:77-81(2020)

 

PubMed=32511316; DOI=10.1101/2020.03.02.972935; PMCID=PMC7239045

Harcourt J.L., Tamin A., Lu X.-Y., Kamili S., Kumar-Sakthivel S., Murray J., Queen K., Tao Y., Paden C.R., Zhang J., Li Y., Uehara A., Wang H.-B., Goldsmith C., Bullock H.A., Wang L.-J., Whitaker B., Lynch B., Gautam R., Schindewolf C., Lokugamage K.G., Scharton D., Plante J.A., Mirchandani D., Widen S.G., Narayanan K., Makino S., Ksiazek T.G., Plante K.S., Weaver S.C., Lindstrom S., Tong S.-X., Menachery V.D., Thornburg N.J.

Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient.

bioRxiv 2020:972935-972935(2020)