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- 保存条件：
详询
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- 英文名：
GELPLATE-clean™ Refill
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
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- 规格：
2x1L
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文献和实验Isolation of Total RNA from Difficult Tissues
techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note
and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
) for 1 minute to elute DNA. 12) For genomic DNA clean up, repeat elution step 10-11 by adding another 15-20 ul Elution Buffer. 2. MicroElute™ DNA Clean-Up Vacuum/Spin Protocol Note: Please read through previous section before using
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