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- 文献和实验
- 技术资料
- 英文名：
ChipShot™ Direct Labeling and Clean-up System
- 供应商：
普洛麦格
- 规格：
25 reactions
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文献和实验, amphibian, insect and plant origins. For many applications elution of the mRNA from the beads is not required as the beads do not interfere with downstream enzymatic reactions. The bead-bound oligo (dT)25 can also function as a primer for RT
Protocol for 5' End Labeling RNA
(available in the KinaseMax™ Kit) or by the following protocol: 3) To the above reaction, add: 125 µl water 15 µl 5.0 M ammonium acetate or 3.0 M sodium acetate 150 µl phenol/chloroform Phenol extract by spinning the tube at room
Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation
Heat to 65 o C for 10 min and cool on ice. Add 14.6 m L of reaction mixture each to Cy3 and Cy5 reactions: Reaction mixture l
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