- $236
- LSM BIO
- 进口
- PVT84334
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pLV3-CMV-Ldlr-EGFP-EF1a-Puro Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
pLV3-CMV-Ldlr-EGFP-EF1a-Puro Plasmid
Catalog No.: PVT84334
pLV3-CMV-Ldlr-EGFP-EF1a-Puro Plasmid
Alias:NM_010700.3;Hlb301;
Host: Mammalian cells,lentivirus
Use(s): Protein expression
Fragment Type: ORF
Fragmented species: Mouse
Prokaryotic resistance: Amp
Screening Markers: Puro
Fluorescence labeling: Green
Promoter: CMV
Competent cells: Stbl3
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
naj2007 请教一下各位老师，在构建过表达载体的时候，VEGF和GFP是不是不可以做成融合蛋白，但是如果用不同的启动子的话，很容易丢失荧光，有没有什么解决方法。 zhujoker 可以做成融合蛋白的，即使是使用不同的启动子的话荧光也不那么容易丢失的，大不了你将荧光的启动子换成强的如CMV，EF1a之类。 kinguangjun 或者你可以用CMV-VEGF-IRES-GFP来做
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