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CellAmp Washing Buffer 2.5 ml × 5
CellAmp Lysis Buffer II 1 ml × 5
DNase I for Direct RNA Prep 200 μl
Stop Solution 250 μl
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文献和实验on the original ELISA principle, this chapter will focus on the two perhaps most useful and routinely performed: the indirect sandwich ELISA, providing high sensitivity and specificity; and the basic direct ELISA, useful when only one antibody to the sample
Rapid Electrical Lysis of Bacterial Cells in a Microfluidic Device
. Applying a direct current (DC) field for cell lysis typically requires field strength of 1–10 kV/cm, which is dependent on the cell type: prokaryotes or eukaryotes. Bubble generation and Joule heating can often be induced under such high field strengths
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
& alternative recipe at end for lysis buffer and IP Dilution Buffer) 2. Add acid-washed glass beads, so there is a 1 to 2mm layer of liquid above the beads. 3. Vortex in the Tomy Mixer. Set it to level 10, mix 8 times for 1 minute each, and put on ice
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