CheKine™ NAD激酶（NADK）活性检测试剂盒（微量

CheKine™ NAD激酶（NADK）活性检测试剂盒（微量法）

产品基础信息

• 货号：KTB1022
• 产品分类：核心产品（细胞分析）
• 检测类型：辅酶相关
• 检测方法：比色法
• 样本兼容性：血清、血浆、组织、细胞、细菌、植物等生物体液

产品规格与组分

• 试剂盒组分：Extraction Buffer、Assay Buffer I、Assay Buffer II、Substrate Mix、Enzyme Mix
• 规格与定价：
  1. 48 T/24 S | ¥939 | 现货（次日发货）
  2. 96 T/48 S | ¥1558 | 现货（次日发货）

核心功能与实验支持

• 精准检测NADK酶活性，支持NAD(H)磷酸化生成NADP(H)的代谢研究
• 提供标准化样品制备流程及酶活计算公式

操作规范与质控

• 实验要求：需使用96孔UV微孔板，Substrate Mix/Enzyme Mix全程冰上操作
• 温控标准：25℃（常规物种）或37℃（哺乳动物）反应体系
• 样本处理：冰上操作，当日完成检测，避免匀浆液反复冻融

存储与运输

• 保存条件：-20℃避光保存，有效期12个月（自生产日期起）
• 运输方式：冰袋（蓝冰）冷链运输

免责声明

警告：本产品仅限研究用途，不适用于人类或临床诊断。使用前请确认不违反当地专利法规，厂商不承担因应用不当导致的连带责任。

生物学背景

NADK（EC 2.7.1.23）作为生物体内唯一催化NAD+生成NADP+的关键酶，通过ATP或poly(P)介导磷酸化反应，在维持NAD(H)/NADP(H)平衡中发挥核心调控作用。

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

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CheKine™ NAD Kinase (NADK) Activity Assay Kit (Micro Method)

Basic Product Information

• Catalog Number:KTB1022
• Product Category:Core Products (Cell Analysis)
• Assay Type:Coenzyme Related
• Assay Method:Colorimetric Method
• Sample Compatibility:Serum, Plasma, Tissue, Cells, Bacteria, Plants, and other biological fluids

Product Specifications and Components

• Kit Components:Extraction Buffer, Assay Buffer I, Assay Buffer II, Substrate Mix, Enzyme Mix
• Specifications and Pricing:
  1. 48 T/24 S | ¥939 | In Stock (Next Day Shipping)
  2. 96 T/48 S | ¥1558 | In Stock (Next Day Shipping)

Core Functions and Experimental Support

• Accurate detection of NADK enzyme activity, supporting metabolic research on NAD(H) phosphorylation to generate NADP(H)
• Provides standardized sample preparation protocols and enzyme activity calculation formulas

Operational Guidelines and Quality Control

• Experimental Requirements:Requires the use of 96-well UV microplates, Substrate Mix/Enzyme Mix must be kept on ice throughout the procedure
• Temperature Control Standards:25℃ (for common species) or 37℃ (for mammals) reaction system
• Sample Handling:Perform on ice, complete the assay on the same day, avoid repeated freeze-thaw cycles of homogenates

Storage and Shipping

• Storage Conditions:Store at -20℃ protected from light, valid for 12 months (from the date of manufacture)
• Shipping Method:Cold chain shipping with ice packs (blue ice)

Disclaimer

Warning:This product is for research use only and is not intended for human or clinical diagnosis. Please ensure that its use does not violate local patent regulations. The manufacturer is not liable for any consequences resulting from improper application.

Biological Background

NADK (EC 2.7.1.23), as the key enzyme in vivo that catalyzes the conversion of NAD+ to NADP+, plays a central regulatory role in maintaining the NAD(H)/NADP(H) balance through ATP or poly(P)-mediated phosphorylation reactions.

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Note: The above information may be subject to changes due to product optimization and upgrades. For specific details, please consult with Yakeyin Company.