CheKine™ 谷胱甘肽S-转移酶（GST）检测试剂盒（微

CheKine™ 谷胱甘肽S-转移酶（GST）检测试剂盒（微量法）

产品概述

货号：KTB1630 | 产品分类：核心产品 | 检测方法：比色法

本试剂盒专为检测血清、血浆、动植物组织/细胞裂解物及其他生物体液中的谷胱甘肽S-转移酶（GST）活性设计，适用于细胞代谢研究领域。

核心特点与优势

• 广泛适用性：支持多种样本类型，包括动植物组织、细胞裂解物及生物体液。
• 高精度分析：提供优化标准曲线及详细样本制备与结果计算指南，确保数据可靠性。
• 操作便捷：试剂盒含预配Assay Buffer、Chromogen及Substrate，简化实验流程。

实验关键注意事项

• 需使用96孔UV微孔板，预实验需筛选2-3个差异样本进行稀释验证线性反应（5分钟内）。
• 反应温度严格控制在25℃（常规物种）或37℃（哺乳动物）。
• 样本处理全程需冰上操作，未检测样本建议-80℃保存（≤1个月）。
• 细胞GST活性检测时，细胞数量需为3-5×10⁶，裂解需使用Assay Buffer研磨/超声处理，禁用细胞裂解液。

保存与运输

• 保存条件：4℃避光保存，有效期12个月（自收货日起）。
• 运输方式：冰袋（蓝冰）低温运输。

产品规格与订购

规格	价格（￥）	货期
48 T/48 S	278	现货（次日发货）
96 T/96 S	426	现货（次日发货）

背景信息

谷胱甘肽S-转移酶（GST）是细胞解毒系统的关键酶，催化亲电化合物与谷胱甘肽（GSH）结合，促进毒性物质排出。其同时具备non-Se GSH-Px活性，可修复氧化损伤的DNA、蛋白质等大分子，在细胞保护中发挥重要作用。

警告：本产品仅限研究使用，不适用于人类或临床诊断。使用须遵守相关专利及法规，对潜在侵权或违规行为不承担责任。

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

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CheKine™ Glutathione S-Transferase (GST) Assay Kit (Micro Method)

Product Overview

Catalog Number: KTB1630 | Product Category: Core Products | Detection Method: Colorimetric Method

This kit is specifically designed for the detection of Glutathione S-Transferase (GST) activity in serum, plasma, animal/plant tissue/cell lysates, and other biological fluids, suitable for the field of cell metabolism research.

Core Features and Advantages

• Wide Applicability: Supports various sample types, including animal/plant tissues, cell lysates, and biological fluids.
• High Precision Analysis: Provides optimized standard curves and detailed sample preparation and result calculation guidelines to ensure data reliability.
• Convenient Operation: The kit includes pre-configured Assay Buffer, Chromogen, and Substrate, simplifying the experimental process.

Key Experimental Considerations

• Use a 96-well UV microplate, and pre-experiments should screen 2-3 different samples for dilution to verify linear reaction (within 5 minutes).
• Reaction temperature should be strictly controlled at 25℃ (for conventional species) or 37℃ (for mammals).
• Sample handling should be performed on ice throughout, and undetected samples are recommended to be stored at -80℃ (≤1 month).
• For cell GST activity detection, the cell number should be 3-5×10⁶, and lysis should be performed using Assay Buffer grinding/ultrasonic treatment, avoiding the use of cell lysis buffer.

Storage and Transportation

• Storage Conditions: Store at 4℃ away from light, valid for 12 months (from the date of receipt).
• Transportation Method: Low-temperature transportation with ice packs (blue ice).

Product Specifications and Ordering

Specification	Price (￥)	Lead Time
48 T/48 S	278	In stock (shipped the next day)
96 T/96 S	426	In stock (shipped the next day)

Background Information

Glutathione S-Transferase (GST) is a key enzyme in the cellular detoxification system, catalyzing the conjugation of electrophilic compounds with glutathione (GSH) to promote the excretion of toxic substances. It also possesses non-Se GSH-Px activity, which can repair oxidative damage to macromolecules such as DNA and proteins, playing an important role in cellular protection.

Warning: This product is for research use only and is not intended for human or clinical diagnosis. Use must comply with relevant patents and regulations, and no liability is assumed for potential infringement or non-compliance.

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Note: The above information may change due to product optimization and upgrades. For specific information, please consult Yakeyin Company.