CheKine™ 线粒体异柠檬酸脱氢酶（ICDHm）活性检测

CheKine™ 线粒体异柠檬酸脱氢酶（ICDHm）活性检测试剂盒（微量法）

产品基础信息

英文名称：CheKine™ Micro Mitochondrial Isocitrate Dehydrogenase（ICDHm）Activity Assay Kit

货号：KTB1250 | 分类：核心产品（细胞分析）

应用领域：细胞代谢检测（三羧酸循环）

试剂盒组分

• Extraction Buffer
• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• ReagentⅣ
• ReagentⅤ

核心特点与优势

• 高效检测：提供简单、快速、精准的ICDHm活性检测方案。
• 广泛兼容性：适配动物组织、植物组织、细胞及真菌等多种样本类型。
• 操作标准化：含详细样本制备流程及酶活计算公式，确保实验可重复性。

检测参数

检测方法：比色法 | 样本类型：不适用

注意事项（关键操作提示）

• 测定管ΔA需控制在0.01-0.3区间，超限需稀释样本。
• 严禁混用不同批次或厂家组分，避免交叉污染。
• 实验全程需低温操作（冰上放置），确保酶活性稳定。
• 推荐基于样本蛋白浓度计算酶活，若按质量计算需额外验证。
• 优先使用新鲜样本提取ICDHm，以保证检测准确性。

存储与运输

保存条件：有效期6个月（具体参考包装清单） | 运输方式：冰袋（蓝冰）冷链

产品规格与交付

规格1：48 T/48 S | 价格：1548元 | 货期：现货（次日发货）

规格2：96 T/96 S | 价格：2306元 | 货期：现货（次日发货）

背景说明

线粒体异柠檬酸脱氢酶（ICDHm）广泛存在于动植物、微生物及细胞线粒体中，催化三羧酸循环中异柠檬酸脱羧生成α-酮戊二酸，并还原NADP+为NADPH。

警告：本产品仅限研究用途，不适用于人类或临床诊断。使用须遵守相关法规，禁止专利侵权或违规操作，我方不承担由此引发的责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

CheKine™ Micro Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity Assay Kit

Basic Product Information

English Name: CheKine™ Micro Mitochondrial Isocitrate Dehydrogenase (ICDHm) Activity Assay Kit

Catalog Number: KTB1250 | Category: Core Products (Cell Analysis)

Application: Cell Metabolism Detection (Tricarboxylic Acid Cycle)

Kit Components

• Extraction Buffer
• ReagentⅠ
• ReagentⅡ
• ReagentⅢ
• ReagentⅣ
• ReagentⅤ

Core Features and Advantages

• Efficient Detection: Provides a simple, rapid, and accurate solution for ICDHm activity detection.
• Broad Compatibility: Compatible with various sample types including animal tissues, plant tissues, cells, and fungi.
• Standardized Operation: Includes detailed sample preparation procedures and enzyme activity calculation formulas to ensure experimental reproducibility.

Detection Parameters

Detection Method: Colorimetric Method | Sample Type: Not Applicable

Precautions (Key Operational Tips)

• The ΔA of the assay tube should be controlled within the range of 0.01-0.3. If it exceeds the limit, the sample should be diluted.
• Do not mix components from different batches or manufacturers to avoid cross-contamination.
• The entire experiment should be performed at low temperature (on ice) to ensure enzyme activity stability.
• It is recommended to calculate enzyme activity based on sample protein concentration. If calculated by mass, additional validation is required.
• Fresh samples are preferred for ICDHm extraction to ensure detection accuracy.

Storage and Transportation

Storage Conditions: Valid for 6 months (refer to the packaging list for details) | Transportation Method: Cold chain with ice packs (blue ice)

Product Specifications and Delivery

Specification 1: 48 T/48 S | Price: 1548 RMB | Lead Time: In stock (shipped the next day)

Specification 2: 96 T/96 S | Price: 2306 RMB | Lead Time: In stock (shipped the next day)

Background Information

Mitochondrial Isocitrate Dehydrogenase (ICDHm) is widely present in animals, plants, microorganisms, and cell mitochondria. It catalyzes the decarboxylation of isocitrate to α-ketoglutarate in the tricarboxylic acid cycle and reduces NADP+ to NADPH.

Warning: This product is for research use only and is not intended for human or clinical diagnosis. Use must comply with relevant regulations. Patent infringement or improper operation is prohibited. We are not responsible for any liability arising from such actions.

Note: The above information may change due to product optimization and upgrades. For specific information, please consult Yakeyin Company.