蓝色预染蛋白分子量标准 (10-250 kDa)

彩色预染蛋白分子量标准：
1. 该产品包含了从10kDa到250kDa共12种高度纯化并预染的重组蛋白质。
2. 适合作为SDS-PAGE和Western的蛋白质分子量标准。
3. 本彩色预染蛋白质分子量标准已经配制在1×SDS-PAGE上样缓冲液中，直接使用，不要煮沸、稀释和加入还原剂处理。
4. 根据上样孔的大小，本彩色预染蛋白质分子量标准通常每次上样5-10微升（5×1.5mm胶孔5μl足够），即可在电泳时、电泳后和转膜后观察到非常清楚的蛋白条带。
使用方法：
1. 室温下解冻后完全溶解并轻轻充分混匀，不要煮沸。
2. 取本产品5µl与实验样品同时进行电泳；建议有条件的实验室在初次使用本产品时可以根据自身的实验条件和实验习惯通过预实验确定合适的上样量，这样可以节约成本，同时获得效果更佳的实验图片。
3. 未使用的彩色预染蛋白分子量标准保存于储存条件，在4°C可放置2个月。
注意事项：
1. 在低浓度胶中，低分子量蛋白会泳于染料前缘。
2. 大分子量蛋白转印时建议延长转膜时间或加高转膜电压，如果效果不好建议微调转膜液配方，减少甲醇用量，添加少量SDS（终浓度不超过0.04%）。
3. 预染蛋白质在不同的缓冲体系下有不同的表观分子量，如果在该缓冲体系中事先用非预染蛋白质标定，可以大致确定蛋白质分子量。

Color pre dyed protein molecular weight standard:
1. This product contains 12 highly purified and pre stained recombinant proteins ranging from 10kDa to 250kDa.
2. Suitable as a protein molecular weight standard for SDS-PAGE and Western blotting.
3. This color pre stained protein molecular weight standard has been prepared in 1 × SDS-PAGE buffer and should be used directly without boiling, diluting, or adding reducing agents.
4. According to the size of the sample well, this color pre stained protein molecular weight standard usually requires 5-10 microliters of sample per loading (5μl for 5 × 1.5mm gel wells is sufficient), and very clear protein bands can be observed during electrophoresis, electrophoresis, and membrane transfer.
Usage method:
1. After thawing at room temperature, completely dissolve and gently mix well, do not boil.
2. Take 5µl of this product and test sample for polyacrylamide gel electrophoresis at the same time; It is recommended that laboratories with conditions determine the appropriate sample size through pre experiments based on their own experimental conditions and habits when using this product for the first time. This can save costs and obtain better experimental images.
3. Unused color pre dyed protein molecular weight standards should be stored under storage conditions and can be left at 4°C for 2 months.
Matters needing attention:
1. In low concentration gels, low molecular weight proteins will swim at the front edge of the dye.
2. When transferring high molecular weight proteins, it is recommended to extend the transfer time or increase the transfer voltage. If the effect is not good, it is recommended to adjust the transfer solution formula, reduce the amount of methanol used, and add a small amount of SDS (final concentration not exceeding 0.04%).
3. Pre stained proteins have different apparent molecular weights in different buffer systems. If non pre stained proteins are calibrated in advance in this buffer system, the protein molecular weight can be roughly determined.