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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80°C
- 保质期：
请咨询
- 英文名：
GFP E. coli Recombinant Protein
- 库存：
1 week
- 供应商：
缔码生物（武汉）科技有限公司
- 规格：
20 ug
| 产品类别 | ECD Proteins |
|---|---|
| 靶点 | HEK293 |
| Uniprot ID | |
| 背景 | A DNA sequence from the destination vector, [PS100019], encoding the turbo GFP fused with myc/DDK tag at c-terminus. |
| 描述 | Purified recombinant green fluorescent protein (GFP) with C-Myc/DDK tag, expressed in HEK293 cells |
| 分子量 | 29 kDa |
| 纯度 | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| 别名 | |
| 标签 | C-Myc/DDK |
| 表达宿主 | HEK293 |
| 组分与重悬 | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol |
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文献和实验Resolving Bottlenecks for Recombinant Protein Expression in E. coli
regarding expression of recombinant proteins in E. coli . However, despite this progress, protein production, primarily of eukaryotic origin, still remains a challenge. The biggest obstacle lies in obtaining large amounts of a given protein in a correctly
Purification of Recombinant Chemokines from E. coli
The majority of chemokines are highly basic, small proteins, with a molecular mass of around 8–10 kDa. Although they do not necessarily have a high level of homology at the primary sequence level, which can be as low as 20
Vectors for the Expression of Recombinant Proteins in E. coli
protein expression. For example, the use of host strains with mutations in cytoplasmic protease genes decreases protein degradation. Hosts that encode tRNAs for codons seldom found in E. coli can successfully express some otherwise difficult to express
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