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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C after receiving vials.
- 库存:
4-5 weeks
- 规格:
20 ug
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文献和实验被单抗的微孔中依次加入基质金属蛋白酶 13 ( MMP-13 ) , 再与 HRP 标记的 MMP-13 抗体结合,形成抗体 - 抗原 - 酶标抗体复合物,经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质金属蛋白酶 13 ( MMP-13 ) 呈正相关。用酶标仪在 450nm 波长下测定吸光度( OD 值),通过标准曲线计算样品中人基质金属蛋白酶 13 ( MMP-13 ) 浓度。 试剂
nm23 Protein Expression by Western Blotting in Patients with Epithelial Ovarian Carcinoma
Western blotting is utilized to detect and quantify the amount of a specific protein in a complex mixture of proteins and at the same time to determine its molecular weight. In particular, by Western blotting it is also possible to detect
UV Absorbance (280 nm) Protein Determination
UV Absorbance (280 nm) � Protein Determination Simple and quick method to accurately quantitate total protein in purified material or approximately quantitate total protein in crude lysates or partial purified material
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