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- 2026年02月03日
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文献和实验and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1 b-mercaptoethanol, 0.02 glusulase and 5ug/ml zymolyase. The suspension is incubated at 37oC
Biotinylated Primer for Detecting Telomerase Activity Without Amplification
to be an attractive new target for designing anti-cancer agents, as well as an important diagnostic marker of human cancer (5 -7 ). For these reasons, it is necessary to develop telomerase assay methods that can be used for the quantitative measurement of telomerase
then recovered and the supernatant discarded) twice with Solution A (1.2 M sorbitol, 50 mM KPO4, pH 7). To spheroplast, cells are resupended in 500 ul of Solution A containing 0.1% b-mercaptoethanol, 0.02% glusulase and 5ug/ml zymolyase. The suspension
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