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Anti-RFa1/Rpa1 | Replication f

actor protein 1 (Saccharomyces cerevisiae)
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  • 询价
  • Agrisera
  • Sweden
  • AS21 4550
  • 2026年01月07日
  • Immunooprecipitation (IP), Western blot (WB)
  • Rabbit
  • Saccharomyces cerevisiae
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Purified, recombinant Rfa1 protein (without a tag) from Saccharomyces cerevisiae, UniProt: P22336

    • 形态

      Serum. Contains 0.05% sodium azide.

    • 保存条件

      Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

    • 克隆性

      Polyclonal

    • 适应物种

      Saccharomyces cerevisiae

    • 供应商

      Agrisera

    • 宿主

      Rabbit

    • 应用范围

      Immunooprecipitation (IP), Western blot (WB)

    • 靶点

      Anti-RFa1/Rpa1 | Replication factor protein 1 (Saccharomyces cerevisiae)

    • 抗体英文名

      Anti-RFa1/Rpa1 | Replication factor protein 1 (Saccharomyces cerevisiae)

    • 抗体名

      Anti-RFa1/Rpa1 | Replication factor protein 1 (Saccharomyces cerevisiae)

    • 规格

      100 µl

    Rfa1 (Replication Factor A protein 1) as part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.

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    图标文献和实验
    相关实验
    • Chromatin immunoprecipitation protocol for Saccharomyces cerevisiae

      double-strand breaks (DSBs) in budding yeast (Saccharomyces cerevisiae ) (Cobb et al., 2003; van Attikum et al., 2004). Back to top Procedure Preculture of yeast cells for ChIP of proteins at DSBs We have used strains with JKM179

    • Replication timing using transient hemimethylation

      . 1992. Telomere-proximal DNA in Saccharomyces cerevisiae is refractory to methyltransferase activity in vivo. Proc. Natl. Acad. Sci. U S A 89: 4062-4065. Hattman, S., C. Kenny, L. Berger, and K. Pratt. 1978. Comparative study of DNA methylation

    • Overview of Protein Microarrays

      , J.S., Ingles, C.J., Hughes, T.R., Parkinson, J., Gerstein, M., Wodak, S.J., Emili, A., and Greenblatt, J.F. 2006. Global landscape of protein complexes in the yeast Saccharomyces cerevisiae. Nature 440:637‐643.

    图标技术资料

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