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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide derived from C-terminal of YFP protein. This peptide is conserved in pGWB541 Vector.
- 形态:
Immunogen affinity purified serum, in PBS pH 7.4, conjugated to DyLight® 594.
- 保存条件:
Store at 4°C for 12-18 months, A preservative may be added for long time storage up to 2 years. Spin briefly the tube before use.
- 克隆性:
Polyclonal
- 适应物种:
C-YFP tagged proteins from Arabidopsis thaliana
- 供应商:
Agrisera
- 宿主:
Rabbit
- 应用范围:
Immunofluorescence (IF), Western blot (WB)
- 靶点:
Anti-C-YFP | C-terminal of YFP - DyLight® 594 Conjugated (40 µg)
- 抗体英文名:
Anti-C-YFP | C-terminal of YFP - DyLight® 594 Conjugated (40 µg)
- 抗体名:
Anti-C-YFP | C-terminal of YFP - DyLight® 594 Conjugated (40 µg)
- 规格:
40 µg
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文献和实验Staining Procedure for Flow Cytometric Detection of Human Cyclins
. Aspirate supernatant. 2. Add 5 ml cold 0.25% Triton ® X-100 in Wash buffer to the cell pellet, vortex and incubate 5 min on ice (or at 4°C). 3. Add 30-40 ml Wash buffer to the above suspension and centrifuge at 200 x g for 10 min. Aspirate
Staining Procedure for Flow Cytometric Detection of Human Cyclins
. Aspirate supernatant. 2. Add 5 ml cold 0.25% Triton ® X-100 in Wash buffer to the cell pellet, vortex and incubate 5 min on ice (or at 4°C). 3. Add 30-40 ml Wash buffer to the above suspension and centrifuge at 200 x g for 10 min. Aspirate
Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor
, Ref. 41001) and the CFP/YFP filter set (e.g.Chroma, Ref. 51017). Confocal microscopy can be performed on a Zeiss LSM510 Axiovert 200M, equipped with a Zeiss Plan-Apochromat 100x/NA=1.4 oil immersion or a Plan-Fluar 100x/NA=1.45 oil immersion
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