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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
inhibition of GSK3 is tomonitor phosphorylation of specific GSK3 substrates in cells (e.g. b-catenin). Alternatively, Wnt inhibitionof GSK3 can be measured by partial purification of cellular GSK3 by ion exchange chromatography andassay of fractions
Determining In Vivo Phosphorylation Sites Using Mass Spectrometry
/) Cutter device (Hamilton, Needle Kel‐F hub (KF), point style 3, gauge 16, cat. no. 90516; plunger assembly N, RN, LT, LTN for model 1702 (25 µl), cat. no. 1122‐01 200‐µl
, which then stimulates osteoclast activity and bone breakdown, freeing calcium into the blood. Parathyroid hormone also increases serum calcium by decreasing renal excretion of calcium and increasing renal excretion of phosphate ion
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