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文献和实验A Proteomics Approach to Identify Redox-Sensitive Proteins
-Rad). 10. CB-X Protein Assay kit (G-Biosciences). 11. Reduction buffer: 50 mM Tris–HCl, pH 8.0, 7 M urea, 2 M thiourea, 1% CHAPS, and 1 mM DTT. 12. 11 cm ReadyStrip IPG strips (pH 4–7) (Bio-Rad). 13. SDS-PAGE running buffer (10×): 250
Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
volume) Transcreener GDP FP Assay kit (Bellbrook Labs, cat. no. 3009‐1K) containing: GDP antibody
Methods for Studying Rodent Intestinal Lipoprotein Production and Metabolism
2 , steps 1 to 8, respectively) 5.0 mCi 3 H‐labeled glycerol Trioleate (PerkinElmer) 1.0 mCi 14 C‐cholesterol (PerkinElmer
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