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文献和实验by the neck, using a sterile metal hook or a bent glass rod, and place the embryo in a 100mm petri dish containing phosphate buffered saline (PBS). Wash several times with PBS by transferring the embryo to fresh petri plates. After removal of all yolk
of resolution. The coverslip glass Although the majority of the coverslips commercially available today are made from optical quality glass and are in the correct thickness range of 0.16 to 0.19 mm, the quality and thickness of the coverslip glass
Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates and Extracts
conditions were 40 mM L-aspartate, 0.4 mM carbamoyl phosphate containing [14 C]carbamoyl phosphate (0.1 μCi μmole -1 ), Tris buffer (0.1 M, pH 8.0), and cell-free extract in a final volume of 200 μl. The reaction was initiated by adding 10 μl of cell-free
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