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文献和实验Establishment of a Primary Culture
and remove the chorioallantoic membrane, exposing the embryo. Identify and carefully remove the embryo by the neck, using a sterile metal hook or a bent glass rod, and place the embryo in a 100mm petri dish containing phosphate buffered
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
by the neck, using a sterile metal hook or a bent glass rod, and place the embryo in a 100mm petri dish containing phosphate buffered saline (PBS). Wash several times with PBS by transferring the embryo to fresh petri plates. After removal of all yolk
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