t-Butyl 2-cyano-2-(3-nitropyri

PCR clean up using 3M sodium acetate and chilled absolute ethanol

  For each 20 ul of PCR product, prepare the following mixture in 1.5 ml Tube. 2 ul of 3M sodium acetate (NaOAc) pH 4.5 40 ul chilled absolute ethanol. Transfer the PCR product (20ul) into the tube containing

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

) and Cs2 CO3 under a nitrogen atmosphere. Conversion to the O 6 ?(benzotriazol?1?yl) ethers occurs within about 10 min for inosine, and within about 60 min for guanosine and 2??deoxyguanosine. Then, for reaction with alcohols, the reaction mixture

Electron Microscopy of Protein-Nucleic Acid Complexes: Uniform Spreading of Flexible Complexes, Staining with a Uniform Thin Layer of Uranyl Acetate,

. Some of the best known examples are the ssb protein of Escherichia coli , the gene 32 protein of phage T4, the gene 5 protein of the M13/fd/f1 filamentous bacterial viruses, and the more recently isolated human replication protein A (1 –4 ). Complexes formed