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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Benzenethiol,dimethyl-, zinc salt (9CI)
- 库存:
98
- 供应商:
北京孚博
- CAS号:
60972-39-6
- 规格:
1g
CAS:60972-39-6
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文献和实验Assembly of Nucleosomal Templates by Salt Dialysis
Because it is convenient to assemble nucleosomal templates through salt dialysis, large amounts of chromatin complexes can be made easily and in a short amount of time. This unit includes instructions for the various salt dialysis schemes (step versus gradient
End-label oligo (20-25 mer) 4 µl 5X T4 Kinase Buffer 5 µl [gamma-32P] ATP (7000 Ci/mmol) 10 µl water + oligo (200 ng) 1 µl T4 Kinase 1. 37 deg C for 1 hour. 2. Heat inactivate at 75 deg C for 10 minutes. 3. Add 1 µl
S1 Nuclease Protection Assay End-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5 µl [gamma-32P] ATP (7000 Ci/mmol)10 µl water + oligo (200 ng)1 µl T4 Kinase1. 37 deg C for 1 hour.2. Heat inactivate at 75 deg C for 10 minutes.3. Add 1 µl glycogen
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