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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
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- 英文名:
Benzenethiol, 2-amino-6-methoxy- (9CI)
- 库存:
98
- 供应商:
北京孚博
- CAS号:
740773-51-7
- 规格:
1g
CAS:740773-51-7
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文献和实验Metabolic Labeling with Amino Acids
are used to study the biosynthesis, processing, intracellular transport, secretion, degradation, and physical?chemical properties of proteins. This unit provides protocols for metabolic labeling of cells with [35 S]?labeled amino acids to provide material
Protein Syntheses in Cell Free Systems
200 mM Tris-HCl, pH 7.5, 1200 mM KCl, 50 mM magnesium acetate and 70 mM -mercaptoethanol 10X solution of 20 amino acids Teflon homogenizer Refrigerated preparative centrifuge Sat. (NH ) SO TBS-M plus 20% (v
depending on mRNA) 1 ml RNAsin 1 ml 1mM amino acid mixture 2 ml mRNA (1 mg or less) 4 ml Met or Cys (1200Ci/mMol,10mCi/ml) inc37℃60',place on ice spot 1 ml on Whatman 1MM,air dry on tin foil boil in 10% TCA 10',sink filters with ice,rinse 2 x quickly
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