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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 克隆性:
Monoclonal
- 适应物种:
null
- 供应商:
OriGene
- 宿主:
Rabbit
- 规格:
100 ul
description:IKB alpha Rabbit monoclonal Antibody
symbol:NFKBIA
synonyms:I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD-3; MAD3; NFKBI
locusid:4792
Concentration:lot specific
sub_category:Primary Antibodies
uniprot_id:P25963
Applications:IHC, IP, WB
CNdelivery:2周
RecommendDilution:WB: 1/1000-1/10000
IHC: 1/20
IP: 1/20
Reactivity:Human, Mouse, Rat
Immunogen:A synthetic peptide of human IKB alpha
Purification:Affinity Purified
Conjugation:Unconjugated
ProteinFamilies:null
ProteinPathways:null
GeneName:NFKB inhibitor alpha
Background:Swiss-Prot Acc.P25963.Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Buffer:50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
isotypes_filter:IgG
predicted_size:Calculated MW: 36 kDa; Observed MW: 39 kDa
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文献和实验confirmation. A. Protein microarray hybridization with 8F1. The OriGene overexpression protein microarray chip was immunostained with the most commonly used 8F1 monoclonal anti-ERCC1 antibody. The positive reactive proteins are highlighted with red arrows
of First Hospital of Harbin Medical University. RPMI 1640 was purchased from GIBCO. Propidium iodide and Rnase were from Sigma Chemical Co. A murine monoclonal antibody against human Bcl-2 and Bax oncoprotein and antimi ce rabbit polyclonal antibody were purchased
/GFP Please read this whole document before you start! Shuttle mutagenesis Clone target gene into vector pHSS6. pHSS6 is in strain R1123; map given below. Delete as much of the polylinker
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