Recombinant TGF beta 1 (Transf

gene_symbol
Description：Recombinant TGF beta 1 (Transforming growth factor beta 1)，Human
Accn
Unipro ID：P01137
Synonyms：Differentiation inhibiting factor, Cartilage-inducing factor, CED, DPD1, IBDIMDE, LAP, TGFB
Species：Human
Amount：20 ug
Delivery time：2周
Expression sequence：MALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS with polyhistidine tag at the C-terminus.
Tags：His Tag (C-term)
PredictedMW：The protein has a calculated MW of 13.7 kDa. The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.
Stability
Bioactivity：Measure by its ability to inhibit the IL-4 dependent proliferation in HT-2 cells. The ED₅₀ for this effect is 5 x 10⁷ IU/mg. Measure by its ability to induce proliferation in MCF-7 cells. The ED₅₀ for this effect is <3.2 ng/mL.
Purity：>98% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background：The transforming Growth Factors beta (TGF beta) family of cytokines are ubiquitous, multifunctional, and essential to survival. They play the central roles in growth, development, inflammation, repair, and host immunity. The mammalian TGF beta isoforms (TGF beta 1, beta 2 and beta 3) are secreted as potential precursors and possess a variety of cell surface receptors and produces at least two mediate signal transductions.