Recombinant Flt-3 Ligand (Fms-

gene_symbol
Description：Recombinant Flt-3 Ligand (Fms-related tyrosine kinase-3 ligand)，Human
Accn
Unipro ID：P49771
Synonyms：FL, FLG3L, FLT3L
Species：Human
Amount：20 ug
Delivery time：2周
Expression sequence：MTQDCSFQHSPISSDFAVKIRELSDYLLQDYPVTVASNLQDEELCGGLWRLVLAQRWMERLKTVAGSKMQGLLERVNTEIHFVTKCAFQPPPSCLRFVQTNISRLLQETSEQLVALKPWITRQNFSRCLELQCQPDSSTLPPPWSPRPLEATAPTA with polyhistidine tag at the C-terminus.
Tags：His Tag (C-term)
PredictedMW：The protein has a calculated MW of 18.6 kDa. The protein migrates as 15-19 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
Stability
Bioactivity：Measure by its ability to induce proliferation in BaF3 cells transfected with mouse Flt-3. The ED₅₀ for this effect is 1.5 x 10⁶ IU/mg.
Purity：>95% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.1 EU per 1 μg of the protein by the LAL method.
Shipping
Background：Fms-related tyrosine kinase-3 ligand (Flt-3 Ligand) is a protein which is encoded by the FLT3LG gene in human. As an important regulator of hematopoiesis, Flt-3 ligand has a tyrosine-protein kinase activity that stimulate the proliferation of hematopoietic progenitor cells of both lymphoid and myeloid origin. Flt-3 ligand can synergistically increase the proliferation of immature progenitors with other cytokines such as GM-CSF, G-CSF, IL-3, EPO, IL-11, IL-12, IL-6 or TPO. Flt-3 ligand is expressed by a variety of hematopoietic progenitor cells including immature hematopoietic and