Recombinant BMP-8b (Bone morph

gene_symbol
Description：Recombinant BMP-8b (Bone morphogenetic protein-8b)，Human
Accn
Unipro ID：P34820
Synonyms：OP-2 (Osteogenic Protein-2), BMP8
Species：Human
Amount：20 ug
Delivery time：2周
Expression sequence：AVRPLRRRQPKKSNELPQANRLPGIFDDVHGSHGRQVCRRHELYVSFQDLGWLDWVIAPQGYSAYYCEGECSFPLDSCMNATNHAILQSLVHLMMPDAVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH with polyhistidine tag at the N-terminus.
Tags：His Tag (N-term)
PredictedMW：The protein has a calculated MW of 16.48 kDa. The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
Buffer：The protein was lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.
Stability
Bioactivity：Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <21.8 ng/mL.
Purity：>98% as determined by SDS-PAGE.
Concentration
Preparation
Endotoxin：<0.01 EU per 1 μg of the protein by the LAL method.
Shipping
Background：Bone Morphogenetic Protein-8B (BMP-8B) is an extracellular multifunctional signaling cytokine that is also a member of the TGF-β family. BMP-8B can bind with the TGF-β receptor and is involved in SMAD protein signal transduction. BMP-8B plays a role in bone and cartilage development. It is expressed in brown adipose tissues and the hypothalamus, which can affect thermogenesis and susceptibility to obesity.