Purified recombinant protein o

gene_symbol：LAMTOR1
Description：Purified recombinant protein of Human late endosomal/lysosomal adaptor, MAPK and MTOR activator 1 (LAMTOR1),Gly2–Pro161, with N-terminal His-PDCD1(Pro21-Val170) tag, expressed in E. coli, 50ug
Accn：NM_017907
Unipro ID：Q6IAA8
Synonyms：C11orf59; p18; p27RF-Rho; PDRO; Ragulator1
Species：Human
Amount：50 ug
Delivery time：现货
Expression sequence：A DNA sequence encoding the region(Gly2–Pro161) of LAMTOR1
Tags：N-His PDCD1(Pro21-Val170)
PredictedMW：34.4 kDa
Buffer：25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Stability：Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity：> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration：>0.05 µg/µL as determined by microplate BCA method
Preparation
Endotoxin
Shipping
Background：As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation.[UniProtKB/Swiss-Prot Function]