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Description:Purified recombinant protein of Human calcium binding atopy-related autoantigen 1 (CBARA1), nuclear gene encoding mitochondrial protein, transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug
Accn:NM_006077
Unipro ID:Q9BPX6
Synonyms:ara CALC; CALC; CBARA1; EFHA3; MPXPS
Species:Human
Amount:50 ug
Delivery time:2周
Expression sequence:A DNA sequence encoding human full-length CBARA1
Tags:N-His
PredictedMW:54.4 kDa
Buffer:25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation
Endotoxin
Shipping
Background:This gene encodes an essential regulator of mitochondrial Ca2+ uptake under basal conditions. The encoded protein interacts with the mitochondrial calcium uniporter, a mitochondrial inner membrane Ca2+ channel, and is essential in preventing mitochondrial Ca2+ overload, which can cause excessive production of reactive oxygen species and cell stress. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2013]
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文献和实验, this expression and functional system has been used to show that the preincubation of Human Odorant Binding Protein 2A decrease the calcium response of OR 17-210 following stimulation by acetophenone and beta ionone.
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
. nos. 1830‐9610) 0.65‐µm pore hydrophilic low‐protein‐binding filter plates (Millipore) Glutathione Sepharose beads (e.g
associates with the recognition protein C1q. The C1 complex circulates in serum as a zymogen and is activated upon binding of C1q to appropriate targets, such as antigen–antibody complexes. This property is used for the purification of C1r and C1s from human
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