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Description:Recombinant protein of human cathepsin D (CTSD)
Accn:NM_001909
Unipro ID:P07339
Synonyms:CLN10; CPSD; HEL-S-130P
Species:Human
Amount:10 ug
Delivery time:2周
Expression sequence:Leu21-Leu412
Tags:C-His
PredictedMW:43.8 kDa
Buffer:Supplied as a 0.2 um filtered solution of 20mM MES, 150mM NaCl, pH 5.5.
Stability:Stable for at least 3 months from date of receipt under proper storage and handling conditions.
Bioactivity
Purity:>95% as determined by SDS-PAGE and Coomassie blue staining
Concentration:lot specific
Preparation
Endotoxin:< 0.1 EU per µg protein as determined by LAL test
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Background:This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease. [provided by RefSeq, Nov 2015]
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文献和实验Purification of Surfactant Protein D (SP-D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
in the same source. We also describe procedures of expressing and purifying a recombinant fragment of human SP-D (rhSP-D) comprising trimeric neck and carbohydrate recognition domains that has been shown to have therapeutic effects in murine models of allergy
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
., Janjua, H., Shastry, R., Ho, C.K., Wang, D., Wang, H., Jiang, M., Montelione, G.T., Stuart, D.I., Owens, R.J., Daenke, S., Schutz, A., Heinemann, U., Yokoyama, S., Bussow, K., and Gunsalus, K.C. 2008. Protein production and purification. Nat. Methods
Isolation of Recombinant DNA Elongation Proteins
This chapter summarizes isolation procedures of four recombinant human proteins crucial for DNA replication: (a) the replicative DNA polymerase (pol) δ, (b) proliferating cell nuclear antigen (PCNA), (c) replication protein A (RP-A), and (d
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