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Description:Recombinant protein of human lipocalin 2 (LCN2), 20 µg
Accn:NM_005564
Unipro ID:P80188
Synonyms:24p3; MSFI; NGAL; p25
Species:Human
Amount:20 ug
Delivery time:现货
Expression sequence:>RC207685 protein sequence Red=Cloning site Green=Tags(s) MPLGLLWLGLALLGALHAQAQDSTSDLIPAPPLSKVPLQQNFQDNQFQGKWYVVGLAGNAILREDKDPQK MYATIYELKEDKSYNVTSVLFRKKKCDYWIRTFVPGCQPGEFTLGNIKSYPGLTSYLVRVVSTNYNQHAM VFFKKVSQNREYFKITLYGRTKELTSELKENFIRFSKSLGLPENHIVFPVPIDQCIDG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tags:C-Myc/DDK
PredictedMW:22.4 kDa
Buffer:25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation:Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Endotoxin
Shipping
Background:This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice. [provided by RefSeq, Sep 2015]
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
. nos. 1830‐9610) 0.65‐µm pore hydrophilic low‐protein‐binding filter plates (Millipore) Glutathione Sepharose beads (e.g
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
a titration of protein A microbeads (as indicated) against a constant amount of Fc‐fusion protein (4 µg). The minimal bead concentration where no free Fc‐fusion remains is selected as optimal (40 µl, the black curve in this case). This figure is reproduced
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
Buffer E 20 mM HEPES-KOH pH 7.8 (1 M stock) 5 mM Potassium Acetate (1 M stock) 0.5 mM MgCl2 (1 M stock) 0.5 mM DTT (1 M stock) Inhibitors: 10 µg
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