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Description:Recombinant protein of human N-acetyltransferase 10 (GCN5-related) (NAT10), transcript variant 1, 20 µg
Accn:NM_024662
Unipro ID:Q9H0A0
Synonyms:ALP; Kre33; NET43
Species:Human
Amount:20 ug
Delivery time:现货
Expression sequence:>RC207082 protein sequence Red=Cloning site Green=Tags(s) MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYKKELGFSSHRK KRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGMCVLQDFEALTPNLLARTVET VEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVVGRFNERFILSLASCKKCLVIDDQLNILPIS SHVATMEALPPQTPDESLGPSDLELRELKESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTV ALTAARGRGKSAALGLAIAGAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPE FNKAVIRVNVFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTG RSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEKWLNDLLCLDCL NITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKNSPNDLQMLSDAPAHHLFCLL PPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRGKKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIA VHPDYQGMGYGSRALQLLQMYYEGRFPCLEEKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNE RPAERLDYLGVSYGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKD FRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMD MIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQ EKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAG PNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tags:C-Myc/DDK
PredictedMW:115.5 kDa
Buffer:25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation:Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Endotoxin
Shipping
Background:The protein encoded by this gene is an RNA cytidine acetyltransferase involved in histone acetylation, tRNA acetylation, the biosynthesis of 18S rRNA, and the enhancement of nuclear architecture and chromatin organization. [provided by RefSeq, Oct 2016]
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文献和实验High Throughput Production of Recombinant Human Proteins for Crystallography
This chapter presents in detail the process used in high throughput bacterial production of recombinant human proteins for crystal structure determination. The core principles are: (1 ) Generating at least 10 truncated constructs
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
, H. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling. Cell 139:610‐622. Hudson, J.R., Jr., Dawson, E.P
Using BLAT to Find Sequence Similarity in Closely Related Genomes
) is used to find genomic sequences that match a protein or DNA sequence submitted by the user. BLAT is typically used for searching similar sequences within the same or closely related species. It was developed to align millions of expressed sequence tags
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