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Description:Recombinant protein of human transgelin 2 (TAGLN2), 20 µg
Accn:NM_003564
Unipro ID:P37802
Synonyms:HA1756
Species:Human
Amount:20 ug
Delivery time:4周
Expression sequence:>RC203473 protein sequence Red=Cloning site Green=Tags(s) MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALY PEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARD DGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tags:C-Myc/DDK
PredictedMW:22.2 kDa
Buffer:25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity:ELISA standard (PMID: 26081836)
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation:Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
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Background:The protein encoded by this gene is similar to the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The specific function of this protein has not yet been determined, although it is thought to be a tumor suppressor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2013]
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
. nos. 1830‐9610) 0.65‐µm pore hydrophilic low‐protein‐binding filter plates (Millipore) Glutathione Sepharose beads (e.g
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
a titration of protein A microbeads (as indicated) against a constant amount of Fc‐fusion protein (4 µg). The minimal bead concentration where no free Fc‐fusion remains is selected as optimal (40 µl, the black curve in this case). This figure is reproduced
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
Buffer E 20 mM HEPES-KOH pH 7.8 (1 M stock) 5 mM Potassium Acetate (1 M stock) 0.5 mM MgCl2 (1 M stock) 0.5 mM DTT (1 M stock) Inhibitors: 10 µg
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