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Description:Recombinant protein of human amyloid P component, serum (APCS), 20 µg
Accn:NM_001639
Unipro ID:P02743
Synonyms:HEL-S-92n; PTX2; SAP
Species:Human
Amount:20 ug
Delivery time:4周
Expression sequence:>RC202802 protein sequence Red=Cloning site Green=Tags(s) MNKPLLWISVLTSLLEAFAHTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFS YNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQ GYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYE IRGYVIIKPLVWV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tags:C-Myc/DDK
PredictedMW:23.2 kDa
Buffer:25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation:Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Endotoxin
Shipping
Background:The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
. nos. 1830‐9610) 0.65‐µm pore hydrophilic low‐protein‐binding filter plates (Millipore) Glutathione Sepharose beads (e.g
DETECTION OF RECOMBINANT PROTEINS BY ELISA
0.5 g BSA QS to 500 ml with H 2O. ELISA Conjugate Diluent PBS, 0.05% Tween-20, 0.1% BSA, 2 µg/ml IgG) Make above ELISA diluent and add 1 mg/ml Bovine Cohn Fraction II IgG
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