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Description:Recombinant protein of human polo-like kinase 1 (Drosophila) (PLK1), 20 µg
Accn:NM_005030
Unipro ID:P53350
Synonyms:PLK; STPK13
Species:Human
Amount:20 ug
Delivery time:4周
Expression sequence:>RC201795 representing NM_005030 Red=Cloning site Green=Tags(s) MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEI SDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLE LHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKT LCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAAS LIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENP LPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDK YGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHL LKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFR TYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Tags:C-Myc/DDK
PredictedMW:68.1 kDa
Buffer:25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Stability:Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Bioactivity:In vitro kinase assay substrate (ELISA) (PMID: 28024153)
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>0.05 µg/µL as determined by microplate BCA method
Preparation:Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Endotoxin
Shipping
Background:The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. [provided by RefSeq, Sep 2015]
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
. nos. 1830‐9610) 0.65‐µm pore hydrophilic low‐protein‐binding filter plates (Millipore) Glutathione Sepharose beads (e.g
-proteins/gsk-3-beta-protein-human-sf9-his.html 糖原合成酶激酶-3β,是 Wnt 信号通路的关键负调控因子,广泛参与代谢、发育、细胞周期及神经功能的调节。 PLK1, Human (HY-P76550) www.medchemexpress.cn/recombinant-proteins/plk1-protein-human-sf9-his.html Polo 样激酶 1,是有丝分裂进程的核心调控者,对中心
Chromatin Assembly Using Drosophila Systems
with the S‐190 Extract Basic Protocol 4: Expression and Purification of the Recombinant Drosophila ACF Basic Protocol 5: Expression and Purification of the Recombinant Drosophila NAP‐1
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