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Size:1 x 96 wells
Curve Range:62.5 pg/ml-4000 pg/ml
Specificity:This kit is used for quantitative detection of rat IL-6
Sensitivity:< 5 pg/ml
Reactivity:Rat
Cross Reactivity:There is no detectable cross-reactivity with other relevant proteins.
Components:96-well plate precoated with anti- rat IL-6 antibody 1Lyophilized recombinant rat IL-6 standard 10 ng/tube x 2Biotinylated anti- rat IL-6 antibody 100 ul(dilution 1:100)Avidin-Biotin-Peroxidase Complex(ABC) 100 ul(dilution 1:100)Sample diluent buffer 30 mlAntibody diluent buffer 12 mlABC diluent buffer 12 mlTMB color developing agent 10 mlTMB stop solution 10 mlPlate seaeler 4 PieceWash Buffer (25x) 20 ml
Gene Symbol:Il6
Species:Rat
Deliveryn Time:3周
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
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