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文献和实验In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
, 2 times (+ 10 min for those non-pos.control slides). (Pos. control slide: in DNase I solution (100µl of 200µg/ml), 10 min., RT. 1x PBS rinse, 2 times in a separate container then combine with other slides.) Wipe around tissue. Make
(+ 10 min for those non-pos.control slides). (Pos. control slide: in DNase I solution (100µl of 200µg/ml), 10 min., RT. 1x PBS rinse, 2 times in a separate container then combine with other slides.) Wipe around tissue. Make up negative
Apoptosis TUNEL Assay (frozen sections)
Citrate, 2 min., 4°C. All slides: 1x PBS rinse, 2 times ( 10 min for those non-pos.control slides). (Pos. control slide: in DNase I solution (100µl of 200µg/ml), 10 min., RT. 1x PBS rinse, 2 times in a separate container then combine
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