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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. Store at -21°C long term. Avoid freeze / thaw cycle.
- 克隆性:
Monoclonal
- 适应物种:
null
- 宿主:
Rabbit
- 规格:
100 ul
description:Phospho-KAP1 (S824) Antibody
symbol:TRIM28
synonyms:KAP 1; KRAB associated protein 1; KRIP1; RNF96; TF1B; TIF1 beta; TIF1B; Trim28; Tripartite motif containing 28
locusid:10155
Concentration:lot specific
sub_category:Primary Antibodies
uniprot_id:Q13263
Applications:IP, WB
CNdelivery:2周
RecommendDilution:WB 1:500~1:2000 IP 1:50
Reactivity:Human
Immunogen:A synthesized peptide derived from human KAP1
Purification:Affinity-chromatography
Conjugation:Unconjugated
ProteinFamilies:null
ProteinPathways:null
GeneName:tripartite motif containing 28
Background:Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes.
Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
isotypes_filter:IgG
predicted_size:110kDa
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文献和实验Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs
Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
Isoelectric Focusing and Immunofixation of Monoclonal Antibodies
Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking
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