- ￥1999
- null
- TA423235
- 2025年07月15日
- Rabbit
- null
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at +4°C short term. Store at -21°C long term. Avoid freeze / thaw cycle.
- 克隆性：
Monoclonal
- 适应物种：
null
- 宿主：
Rabbit
- 规格：
100 ul
description：Atg4A Antibody
symbol：ATG4A
synonyms：Apg4a; ATG4A; Atg4al; Autl2; AUT like 2 cysteine endopeptidase; Autophagin-2; Autophagin 2; Autophagy related 4A cysteine peptidase
locusid：115201
Concentration：lot specific
sub_category：Primary Antibodies
uniprot_id：Q8WYN0
Applications：IHC, IP, WB
CNdelivery：2周
RecommendDilution：WB 1:500~1:2000 IHC 1:50~1:200 IP 1:50
Reactivity：Human, Mouse, Rat
Immunogen：A synthesized peptide derived from human Atg4A
Purification：Affinity-chromatography
Conjugation：Unconjugated
ProteinFamilies：null
ProteinPathways：null
GeneName：autophagy related 4A cysteine peptidase
Background：The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.
Buffer：Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
isotypes_filter：IgG
predicted_size：45kDa
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文献和实验指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs
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