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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
- 克隆性:
Monoclonal
- 适应物种:
null
- 宿主:
Rabbit
- 规格:
100 ul
description:Argonaute 2 Antibody
symbol:AGO2
synonyms:Ago 2; Argonaute 2; dAgo2; eIF2C 2; hAgo2; PPD
locusid:27161
Concentration:lot specific
sub_category:Primary Antibodies
uniprot_id:Q9UKV8
Applications:FC, ICC/IF, IHC, IP, WB
CNdelivery:2周
RecommendDilution:WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
Reactivity:Human, Mouse, Rat
Immunogen:A synthesized peptide derived from human Argonaute 2
Purification:Affinity-chromatography
Conjugation:Unconjugated
ProteinFamilies:null
ProteinPathways:null
GeneName:argonaute 2, RISC catalytic component
Background:Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
isotypes_filter:IgG
predicted_size:97kDa
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文献和实验Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs
Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
Isoelectric Focusing and Immunofixation of Monoclonal Antibodies
Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking
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