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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. Store at -21°C long term. Avoid freeze / thaw cycle.
- 克隆性:
Monoclonal
- 适应物种:
null
- 宿主:
Rabbit
- 规格:
100 ul
description:CD45 Antibody
symbol:PTPRC
synonyms:B220; CD45; GP180; LCA; PTPRC; T200 leukocyte common antigen
locusid:5788
Concentration:lot specific
sub_category:Primary Antibodies
uniprot_id:P08575
Applications:FC, ICC/IF, IHC, WB
CNdelivery:2周
RecommendDilution:WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:100 FC 1:20
Reactivity:Human
Immunogen:A synthesized peptide derived from human CD45
Purification:Affinity-chromatography
Conjugation:Unconjugated
ProteinFamilies:null
ProteinPathways:null
GeneName:protein tyrosine phosphatase, receptor type C
Background:Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
Buffer:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
isotypes_filter:IgG
predicted_size:150kDa
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文献和实验Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs
Polyclonal Antibody Production
, if desired this is best accomplished by Protein A Affinity Chromatography. 上一篇:make an Antibody against a Peptides 下一篇:Immunization Protocol for Monoclonal Antibody Production
Isoelectric Focusing and Immunofixation of Monoclonal Antibodies
Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking
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