60108-706赛默飞HyperSep C8固相萃取小柱，

Protocol for Trichloroacetic Acid (TCA) Precipitation

and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish

Pierce推出更快更彻底的新品去垢剂

三次。加入样品，孵育10分钟。离心后，即可收集到无去垢剂的样品。 对于高浓度的蛋白或多肽样品(>100 µg/mL)，Thermo Scientific Pierce也提供去垢剂去除树脂，它能够与10µL-1mL样品中的高浓度去垢剂高效结合并去除，而样品损失极少。 下表显示了样品(0.1mL，含有100µg BSA 和去垢剂)经过0.5mL去垢剂去除树脂处理后，去垢剂的去除百分比以及BSA的回收率。 去垢

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth