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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
干燥;-80℃储存
- 英文名:
Oxotremorine M iodide
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
3854-04-4
- 规格:
O655199-1ml
英文名:Oxotremorine M iodide
纯度或规格:10mM in DMSO
SPU:O655199
CAS:3854-04-4
存储温度:干燥;-80℃储存
运输条件:特低温运输
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文献和实验Purification of recombinant sBRF M166L
, add ZnS04 to a concentration of 20 micromolar, and add all protease inhibitors to 1X concentration. For each dialysis step, use a buffer to sample ratio of 40:1. Dialyze 4 times for four hours each time into decreasing amounts of Urea: 4M, then 2M
Purification of recombinant sBRF M166L
with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml)and grow at 30 deg.for four hours.Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g).Resuspend the cell
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
5ml 10% 20mM EDTA 4ml 250mM 10mM Tris 8 0.5ml 1M 0.8M NH4OAc 5ml 8M 10ug/ml glycogen 50ul 10mg/ml 5)P/CHCl3 ext 6)EtOH ppt 7)PAGE: Resuspend carefully in 8ul sequencing sample buffer (5''vortex, 5'' 60
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