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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
HUP30
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
312747-21-0
- 规格:
H656519-1ml
英文名:HUP30
纯度或规格:10mM in DMSO
SPU:H656519
CAS:312747-21-0
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验RNase-Free DNase 30 Kunitz Units - - - GlutaMAX - 2mM - - Non-Essential Amino Acids - 1× - - HEPES - 10mM - - N2 - 1× - - B27 - 1× - - Heparin - 4μg/ml
,so just grow the cells at 30 or 37 ℃ when making BL21 competent cells.However,it has been suggested that the efficiency of BL21 prepared using Inoue method may be improved by treating it with DTT before freezing (add to 3.5% v/v of a 2.2M DTT,10mM KAc pH
strains may work better than others (DH5alpha works well in my hand).Note also that some cells (e.g.HB101)has greater recombination activity than others. This method doesn't appear to work with BL21,so just grow the cells at 30 or 37 ℃
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