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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
Piperaquine phosphate
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
85547-56-4
- 规格:
P426376-1ml
英文名:Piperaquine phosphate
纯度或规格:2mM in Water
SPU:P426376
CAS:85547-56-4
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验it should an 18-22-mer with an annealing temperature of around 56℃, where a G/C = 4℃ and an A/T = 2℃.Protocol1. Place approx. 1ug of total RNA (~10ng polyA RNA) in an RNase-free eppendorf, make up to 10μl with pure water. Add 1μl of T17AP (0.5ug/ul).2. Heat
The protocol given uses SuperScript II reverse transcriptase (Life Technologies) and Taq polymerase from Boehringer Mannheim. You will need to adjust the reagents according to the enzymes you use. Reagents: Sterile water (high purity, RNase-free)
: 35.5ul pure water 5ul 10 x PCR buffer (Boehringer Mannheim) 5ul 500uM dNTPs (some enzymes require a higher concentration of dNTPs (2mM), for example Pfu polymerase (though I'd recommend against the use
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