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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
L-Lysine
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
56-87-1
- 规格:
L424803-1ml
英文名:L-Lysine
纯度或规格:Moligand™, 10mM in Water
SPU:L424803
CAS:56-87-1
存储温度:-80℃储存
运输条件:特低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验Separation of sea urchin blastomeres
the fertilization envelope. The cells were then be allowed to cleave, at which point they were be introduced into a hypertonic seawater solution. This caused the cells to shrink and break apart. Once the majority of the cells had separated, the water was replaced
105/ml的密度接种于预先经L-多聚赖氨酸处理的96孔和24孔培养板,其中24孔板中预先放置有盖玻片。细胞维持在培养液中,置入5%CO2,饱和湿度的培养箱中培养,24h后全换液,接种当天为第一天,每3d半量更换培养液一次,培养第3天时加入阿糖胞苷,终浓度为10μmol/L,以抑制神经胶质细胞的过度增殖,24h后更换全部培养液继续培养,第10天进行细胞造模,开始实验。 也可以用PC12细胞株,常规培养。 Aβ产物诱导海马神经细胞,建立细胞模型:选择Aβ25-35片段,用三蒸水配制成100μmol
ORNL MICROARRAY HYBRIDIZATION PROTOCOLS
slowly down onto array. If bubbles form, do not attempt to "push" them out. This will damage your array. Hybridize the array in a humidified container overnight (16-18 hours) in a 42°C water bath. (floating is usually OK, but can be placed
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